PpPIF-1: first isolated full-length PIF-like element from the bamboo Phyllostachys pubescens.
PpPIF-1: first isolated full-length PIF-like element from the bamboo Phyllostachys pubescens.
复制标题
PpPIF-1：第一个从竹毛竹中分离出全长 PIF 样元件。
DOI：
10.4238/2012.april.3.3
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
D. Tang
中科院分区：
文献类型：
--
作者：
Mingbing Zhou;X. M. Liu;D. Tang
PIF样元件是最近发现的广泛分布的DNA转座子超家族的第一个描述的成员，命名为PIF/Harbinger。完整的和部分的类PIF元件已经从数百种植物中分离出来。之前，我们在竹亚科中鉴定了139个部分PIF样转座酶，其中3个来自毛竹。在这里，我们报告的第一个全长PIF样元件（PpPIF-1）的鉴定和分离，从P. pubescens;鉴定是通过染色体步移，基于修改后的磁性富集程序，允许有效克隆侧翼序列长达3 kb的长度。PpPIF-1基因全长5953 bp，含有20 bp的不完全反向末端重复序列和3 bp的靶位点重复序列。该元件包含两个开放阅读框，一个编码推定的转座酶，包括来自植物的PIF/Harbinger元件典型的完整DDE结构域，另一个编码DNA结合蛋白。在开放阅读框中有7个终止密码子和2个移码突变，可能是由于垂直失活。
PIF-like elements are the first-described members of a recently discovered and widespread superfamily of DNA transposons, named PIF/Harbinger. Complete and partial PIF-like elements have been isolated from hundreds of plant species. Previously, we identified 139 partial PIF-like transposases in the Bambusoideae, of which three were from the bamboo species Phyllostachys pubescens. Here we report identification and isolation of the first full-length PIF-like element (PpPIF-1) from P. pubescens; identification was made by chromosome walking, based on a modified magnetic enrichment procedure that allows efficient cloning of flanking sequences up to 3 kb in length. PpPIF-1 is 5953 bp in length, with 20-bp imperfect inverted terminal repeats and 3-bp target site duplications. This element contains two open reading frames, one encoding a putative transposase, including the complete DDE-domain typical of PIF/Harbinger elements from plants, and the other encoding a DNA-binding protein. There are seven termination codons and two frameshift mutations in the open reading frames, probably due to vertical inactivation.
影响因子：
11.4
作者：
Hartl, DL;Lozovskaya, ER;Lohe, AR
通讯作者：
Lohe, AR
影响因子：
10.7
作者：
Casola, Claudio;Lawing, A. Michelle;Feschotte, Cedric
通讯作者：
Feschotte, Cedric