Gamma Radiation-Induced Damage in the Zinc Finger of the Transcription Factor IIIA.
Gamma Radiation-Induced Damage in the Zinc Finger of the Transcription Factor IIIA.
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伽马辐射诱导的转录因子 IIIA 锌指损伤
DOI：
10.1155/2016/1642064
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发表时间：
2016
影响因子：
3.8
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Zhang X;Miao Y;Hu X;Min R;Liu P;Zhang H
锌指基序是蛋白质的一种元素，可以特异性地识别和结合DNA。因为它们含有多个半胱氨酸残基，锌指基序具有氧化还原性质。电离辐射在生物体内产生多种自由基。因此，锌指基序可能是电离辐射的靶点。研究了γ辐射对锌指蛋白转录因子Ⅲ A（TFIIIA）锌指基序的影响。TFIIIA暴露于来自60 Co源的不同伽马剂量。剂量率分别为0.20戈伊/min和800戈伊/h。使用电泳迁移率变动测定法测定TFIIIA中锌指基序的结合能力。我们发现，1000戈伊的γ射线损害的功能的锌指基序在TFIIIA。辐射诱导的锌指损伤的位点是半胱氨酸残基和锌（II）离子的巯基。硫醇基团被氧化形成二硫键，锌（II）离子被还原为锌原子。这些结果表明锌指基序是γ辐射的靶域，其可通过损伤TFIIIA中的锌指基序来降低5S rRNA表达。
A zinc finger motif is an element of proteins that can specifically recognize and bind to DNA. Because they contain multiple cysteine residues, zinc finger motifs possess redox properties. Ionizing radiation generates a variety of free radicals in organisms. Zinc finger motifs, therefore, may be a target of ionizing radiation. The effect of gamma radiation on the zinc finger motifs in transcription factor IIIA (TFIIIA), a zinc finger protein, was investigated. TFIIIA was exposed to different gamma doses from 60Co sources. The dose rates were 0.20 Gy/min and 800 Gy/h, respectively. The binding capacity of zinc finger motifs in TFIIIA was determined using an electrophoretic mobility shift assay. We found that 1000 Gy of gamma radiation impaired the function of the zinc finger motifs in TFIIIA. The sites of radiation-induced damage in the zinc finger were the thiol groups of cysteine residues and zinc (II) ions. The thiol groups were oxidized to form disulfide bonds and the zinc (II) ions were indicated to be reduced to zinc atoms. These results indicate that the zinc finger motif is a target domain for gamma radiation, which may decrease 5S rRNA expression via impairment of the zinc finger motifs in TFIIIA.
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DOI：
10.1016/j.bbrc.2005.04.170
发表时间：
2005-07-08
影响因子：
3.1
作者：
Feng, W;Cai, J;Kang, YJ
通讯作者：
Kang, YJ
影响因子：
4.8
作者：
Kröncke, KD
通讯作者：
Kröncke, KD
影响因子：
3.9
作者：
Larabee, JL;Hocker, JR;Hanas, JS
通讯作者：
Hanas, JS
影响因子：
3.5
作者：
Witkiewicz-Kucharczyk, A;Bal, W
通讯作者：
Bal, W
影响因子：
4.8
作者：
Brady, KL;Ponnampalam, SN;Setzer, DR
通讯作者：
Setzer, DR