FIBER TYPE SPECIFIC DISTRIBUTION OF STRESS PROTEINS IN RAT SKELETAL MUSCLE
FIBER TYPE SPECIFIC DISTRIBUTION OF STRESS PROTEINS IN RAT SKELETAL MUSCLE
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大鼠骨骼肌中应激蛋白的纤维类型特异性分布
DOI：
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
H. Matsumoto
中科院分区：
文献类型：
--
作者：
Y. Oishi;A. Ishihara;H. Ifuku;K. Taniguchi;H. Matsumoto
为了确定非应激大鼠骨骼肌中是否存在纤维类型特异性热休克蛋白（HSP或应激蛋白）的表达，我们对成年雌性Sprague-Dawley大鼠的内侧腓骨进行了免疫组织化学分析。采用5种抗肌球蛋白重链（MHC）抗体对纤维类型进行分类，2种抗hsp抗体对纤维类型特异性表达进行分析。低温恒温器连续切割10个ƒÊm厚截面，用抗mhc或抗hsp 60和72抗体孵育，然后用生物素化的抗小鼠二抗和亲和素-生物素复合物溶液孵育。使用过氧化物酶DAB底物试剂盒（Vector SK-4100）或BCIP/NBT溶液观察每种纤维类型的免疫反应。通过5种抗mhc抗体，将纤维分为慢型I、快型IIA、IIX和IIB 4种类型。Anti-HSP 72抗体与许多（但不是全部）I型和IIA型纤维反应，而Anti-HSP 60抗体仅与I型纤维反应。IIX型和IIB型纤维对抗hsp 60或72抗体均未表现出免疫反应性。这些结果表明，在非应激大鼠骨骼肌中，HSP 60蛋白的表达与I型MHC的表达有关，HSP 72蛋白的表达可能与I型和ha型MHC的表达有关。(日本。期刊。关键词：应激蛋白，热休克蛋白（HSP），肌球蛋白重链（MHC），纤维类型，免疫组织化学
To determine whether fiber type-specific expression of heat shock protein (HSP, or stress protein) occurs in unstressed rat skeletal muscle, the medial gastrocnemius of adult female Sprague-Dawley rats was subjected to immunohistochemical analysis. Antibodies against 5 types of anti-myosin heavy chain (MHC) were used to classify the type of fibers, and 2 types of anti-HSP antibodies were employed to analyze the fiber type-specific expression. Serial cross-sections of 10 ƒÊm thick cut by a cryostat were incubated with primary anti-MHC or anti-HSP 60 and 72 antibodies, followed by biotinylated secondary anti-mouse antibodies, and avidin-biotin complex solution. A peroxidase DAB substrate kit (Vector SK-4100) or BCIP/NBT solution was used to visualize the immunoreaction of each fiber type. By using the 5 types of anti-MHC antibodies, fibers were classified into 4 types : slow-type I, fast types IIA, IIX, and IIB. Anti-HSP 72 antibody reacted with many, but not all, type I and IIA fibers, whereas anti-HSP 60 antibody reacted specifically with type I fibers. Neither type IIX nor IIB fibers showed immunoreactivity with anti-HSP 60 or 72 antibodies. These results suggest that the expression of HSP 60 protein is related to that of type I MHC, and that the expression of HSP 72 protein may be related to that of types I and ha MHC, in unstressed rat skeletal muscle. (Jpn. J. Phys. Fitness Sports Med. 1998, 47: 87•`92) key words : stress protein, heat shock protein (HSP), myosin heavy chain (MHC), fiber type, immunohistochemistry
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人类 HSP70 基因在大鼠生肌细胞系中的稳定表达可提供针对内毒素的保护。
DOI：
10.1152/ajpcell.1996.270.4.c1017
发表时间：
1996
期刊：
The American journal of physiology.
影响因子：
--
作者：
Chi,SH;Mestril,R
通讯作者：
Mestril,R
DOI：
10.1249/00005768-199405001-00755
发表时间：
1994-05
期刊：
The American journal of physiology
影响因子：
--
作者：
K. Kregel;R. Skidmore;J. Gutierrez;V. Guerriero
通讯作者：
K. Kregel;R. Skidmore;J. Gutierrez;V. Guerriero
影响因子：
7.4
作者：
SALO, DC;DONOVAN, CM;DAVIES, KJA
通讯作者：
DAVIES, KJA
影响因子：
15.9
作者：
MARBER, MS;MESTRIL, R;DILLMANN, WH
通讯作者：
DILLMANN, WH
DOI：
10.1152/ajpcell.1996.270.2.c429
发表时间：
1996-02-01
影响因子：
5.5
作者：
Musch, MW;Ciancio, MJ;Chang, EB
通讯作者：
Chang, EB