Isolation and characterization of cDNA clones encoding Photosystem I subunits with molecular masses 11.0, 10.0 and 8.4 kDa from Chlamydomonas reinhardtii
Isolation and characterization of cDNA clones encoding Photosystem I subunits with molecular masses 11.0, 10.0 and 8.4 kDa from Chlamydomonas reinhardtii
复制标题
莱茵衣藻编码光系统 I 亚基（分子量为 11.0、10.0 和 8.4 kDa）的 cDNA 克隆的分离和表征
DOI：
--
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
J. Rochaix
中科院分区：
文献类型：
--
作者：
L. Franzen;G. Frank;H. Zuber;J. Rochaix
使用基因特异性寡核苷酸作为探针，分离编码莱茵衣藻的三个光系统 I 亚基（表观分子量分别为 13、5 和 3 kDa（类囊体多肽 28、35 和 37；分别为 P28、P35 和 P37））的 cDNA 克隆摘要。这些寡核苷酸的序列是从蛋白质的N端氨基酸序列推导出来的。对 cDNA 进行测序并用于探测 Southern 和 Northern 印迹。 Southern印迹分析表明这些蛋白质是由单拷贝基因编码的。三种成分的 mRNA 大小分别为 960 (P28)、1120 (P35) 和 790 (P37) 个核苷酸。 cDNA 的开放阅读框与蛋白质的 N 端氨基酸序列之间的比较表明，新生多肽具有 N 端转运序列，该序列被去除以产生 11.0 (P28)、10.0 (P35) 和 8.4 (P37) kDa 的成熟蛋白质。对推导的蛋白质序列的分析表明，P28 和 P35 是外在膜蛋白，而 P37 跨越类囊体膜。所有三种蛋白质都具有短的转运肽，可能将它们引导至类囊体膜的基质侧。
SummarycDNA clones encoding three photosystem I subunits of Chlamydomonas reinhardtii with apparent molecular masses 13, 5 and 3 kDa (thylakoid polypeptides 28, 35 and 37; P28, P35 and P37, respectively) were isolated using gene specific oligonucleotides as probes. The sequences of these oligonucleotides were deduced from the N-terminal amino acid sequences of the proteins. The cDNAs were sequenced and used to probe Southern and Northern blots. The Southern blot analysis indicates that the proteins are encoded by single-copy genes. The mRNA sizes of the three components are 960 (P28), 1120 (P35) and 790 (P37) nucleotides. Comparison between the open reading frames of the cDNAs and the N-terminal amino acid sequences of the proteins indicates that the nascent polypeptides possess N-terminal transit sequences that are removed to give mature proteins of 11.0 (P28), 10.0 (P35) and 8.4 (P37) kDa. Analysis of the deduced protein sequences suggests that P28 and P35 are extrinsic membrane proteins and that P37 spans the thylakoid membrane. All three proteins have short transit peptides that probably route them to the stromal side of the thylakoid membrane.
影响因子：
5.6
作者：
EISENBERG, D;SCHWARZ, E;WALL, R
通讯作者：
WALL, R
DOI：
10.1073/pnas.84.3.749
发表时间：
1987-02-01
影响因子：
11.1
作者：
MAYFIELD, SP;RAHIRE, M;ROCHAIX, JD
通讯作者：
ROCHAIX, JD