Prostaglandin E2 production by dispersed canine fundic mucosal cells. Contribution of macrophages and endothelial cells as major sources.
Prostaglandin E2 production by dispersed canine fundic mucosal cells. Contribution of macrophages and endothelial cells as major sources.
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前列腺素 E2 由分散的犬胃底粘膜细胞产生。
DOI：
10.1172/jci114330
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Soll,AH
中科院分区：
文献类型：
--
作者：
Chen,MC;Sanders,MJ;Amirian,DA;Thomas,LP;Kauffman,G;Soll,AH
Endogenous prostaglandins (PGs) influence resistance of the gastric mucosa to injury, but the source of PGs is unknown. Using radioimmunoassay, we studied PG production by dispersed canine fundic mucosal cells. PGE2 production, stimulated by bradykinin, epidermal growth factor, zymosan, and calcium ionophore, was greater in the small-cell elutriator fraction (SCEF) than in the medium and large cell fractions, which contained mucous, chief, and parietal cells. Linear density gradients of SCEF cells revealed maximal PGE2 production in cells of light density. Mast, endocrine, and endothelial cells did not account for this PGE2 production. Macrophages, identified by uptake of acetylated-LDL, immunoreactivity with antibodies to the human Ia antigen, and phagocytosis of fluorescent latex particles, were enriched in the SCEF and correlated with PGE2 production in the density gradient. Magnetic separation of cells in the SCEF-ingesting iron particles enriched PGE2 production. Fractions enriched in endothelial cells present in intact capillary fragments, but depleted of macrophages, also produced PGE2. Regulation of PGE2 production differed among cell types. Fibroblasts were easily cultured from submucosa, but were not detected in the SCEF. We conclude that macrophages and capillary endothelial cells are major producers of PGE2 in the canine fundic mucosa.Images
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影响因子：
4.8
作者：
M. A. Clark;D. Littlejohn;T. M. Conway;S. Mong;S. Steiner;S. Crooke
通讯作者：
S. Crooke
DOI：
--
发表时间：
1983
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Hojvat,SA;Musch,MW;Miller,RJ
通讯作者：
Miller,RJ
在分离的犬壁细胞中，卡巴胆碱释放前列腺素 E2 和 [14C]花生四烯酸。
DOI：
--
发表时间：
1987
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Payne,NA;Gerber,JG
通讯作者：
Gerber,JG
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DOI：
10.1152/ajpgi.1982.242.1.g21
发表时间：
1982
期刊：
The American journal of physiology
影响因子：
--
作者：
R. Levine;K. R. Kohen;E. Schwartzel;C. Ramsay
通讯作者：
C. Ramsay
培养的兔胃上皮细胞产生前列腺素I2。
影响因子：
29.4
作者：
Koozi Matuoka;Masanobu Tanaka;Youji Mitsui;Sei
通讯作者：
Sei