High Diversity and Functional Potential of Undescribed "Acidobacteriota" in Danish Wastewater Treatment Plants.
High Diversity and Functional Potential of Undescribed "Acidobacteriota" in Danish Wastewater Treatment Plants.
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DOI：
10.3389/fmicb.2021.643950
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发表时间：
2021
影响因子：
5.2
通讯作者：
Nielsen PH
中科院分区：
文献类型：
--
作者：
Kristensen JM;Singleton C;Clegg LA;Petriglieri F;Nielsen PH
水资源回收设施中的微生物群落包含大量多样性的特征不明确的谱系，这些谱系可能具有未描述的过程关键功能。近年来，在丹麦的活性污泥污水处理厂（WWTP）中发现了丰富的“酸杆菌门”类群，本文对其多样性、分布和功能潜力进行了研究。通过对37个污水处理厂的16S rRNA基因全长参考数据集和扩增子测序调查，确定了“酸杆菌”分类群。该门的成员种类繁多，分属于14科，其中8科完全没有特征，缺乏型菌株。一些谱系是丰富的，相对丰度高达5%的微生物群落。来自19个污水处理厂的50个高质量“酸杆菌”元基因组组装基因组（MAGs）的基因组注释和代谢重建显示出高度的代谢多样性，并可能参与氮、磷的去除和铁的还原。荧光原位杂交（FISH）利用新设计的探针揭示了不同形态的细胞，主要位于活性污泥絮凝体内。FISH结合拉曼显微光谱分析揭示了探针定义的Holophagaceae， Thermoanaerobaculaceae和Vicinamibacteraceae以及占位名称为midas_f_502， midas_f_973和midas_f_1548的细胞的生态生理特征。这些谱系的成员有可能成为多磷酸盐积累生物（PAOs），因为在细胞内观察到关键化合物多磷酸盐和糖原的储存。
Microbial communities in water resource recovery facilities encompass a large diversity of poorly characterized lineages that could have undescribed process-critical functions. Recently, it was shown that taxa belonging to “Acidobacteriota” are abundant in Danish full-scale activated sludge wastewater treatment plants (WWTP), and here we investigated their diversity, distribution, and functional potential. “Acidobacteriota” taxa were identified using a comprehensive full-length 16S rRNA gene reference dataset and amplicon sequencing surveys across 37 WWTPs. Members of this phylum were diverse, belonging to 14 families, eight of which are completely uncharacterized and lack type strains. Several lineages were abundant, with relative abundances of up to 5% of the microbial community. Genome annotation and metabolic reconstruction of 50 high-quality “Acidobacteriota” metagenome-assembled genomes (MAGs) from 19 WWTPs showed high metabolic diversity and potential involvement in nitrogen and phosphorus removal and iron reduction. Fluorescence in situ hybridization (FISH) using newly-designed probes revealed cells with diverse morphologies, predominantly located inside activated sludge flocs. FISH in combination with Raman microspectroscopy revealed ecophysiological traits in probe-defined cells from the families Holophagaceae, Thermoanaerobaculaceae, and Vicinamibacteraceae, and families with the placeholder name of midas_f_502, midas_f_973, and midas_f_1548. Members of these lineages had the potential to be polyphosphate-accumulating organisms (PAOs) as intracellular storage was observed for the key compounds polyphosphate and glycogen.
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影响因子：
4.4
作者：
Caccavo, F;Frolund, B;Nielsen, PH
通讯作者：
Nielsen, PH
影响因子：
3.7
作者：
Farag IF;Davis JP;Youssef NH;Elshahed MS
通讯作者：
Elshahed MS
DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
4.4
作者：
Hernández MA;Mohn WW;Martínez E;Rost E;Alvarez AF;Alvarez HM
通讯作者：
Alvarez HM
影响因子：
3.7
作者：
Ivanova, Anastasia A.;Zhelezova, Alena D.;Dedysh, Svetlana N.
通讯作者：
Dedysh, Svetlana N.