Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field isolates in Korea.
Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field isolates in Korea.
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DOI：
10.1007/s00705-010-0892-9
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发表时间：
2011-04
影响因子：
2.7
通讯作者：
Park BK
中科院分区：
文献类型：
--
作者：
Park SJ;Kim HK;Song DS;Moon HJ;Park BK
猪流行性腹泻病毒(PEDV)自1992年在韩国首次发现以来，已引起严重的肠道疾病。在这项研究中，我们调查了分子流行病学，显示了遗传多样性，并分析了韩国PEDV野毒株与其他PEDV参考毒株的系统发育关系。对M和ORF3全基因的遗传分析表明，每个PEDV组都有几个独特的特征，这表明特定的PEDV组可能通过特定的核苷酸差异而与其他PEDV组区分开来。特别是，ORF3基因分析可以用于区分疫苗型和野生型PEDV。序列和系统发育分析表明，最近流行的韩国PEDV野毒株与中国野毒株有密切的亲缘关系，与欧洲毒株和韩国使用的疫苗株在基因上存在差异。这些结果提出了一个问题，即在韩国是否需要一种新型的PEDV疫苗来更有效地预防PEDV感染。
Porcine epidemic diarrhea virus (PEDV) has caused enteric disease with devastating impact since the first identification of PEDV in 1992 in Korea. In this study, we investigated molecular epidemiology, showed genetic diversity, and analyzed phylogenetic relationships of Korean PEDV field isolates with other PEDV reference strains. Genetic analysis of the complete M and ORF3 genes showed that each PEDV group had several unique characteristics, and this indicated that specific groups of PEDVs may be differentiated from the other PEDVs by specific nucleotide differences. Especially, ORF3 gene analysis can be used for discrimination between vaccine and wild-type PEDVs. Sequence and phylogenetic analysis showed that recent, prevalent Korean PEDV field isolates have close relationships to Chinese field strains and differ genetically from European strains and vaccine strains used in Korea. These results raise questions as to whether a new type of PEDV vaccine may be necessary for preventing PEDV infection more effectively in Korea.
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