Organ-on-a-chip technology: a novel approach to investigate cardiovascular diseases.
Organ-on-a-chip technology: a novel approach to investigate cardiovascular diseases.
复制标题
器官片技术：一种研究心血管疾病的新方法。
DOI：
10.1093/cvr/cvab088
复制
发表时间：
2021-12-17
影响因子：
10.8
通讯作者：
Maegdefessel L
中科院分区：
文献类型：
--
作者：
Paloschi V;Sabater-Lleal M;Middelkamp H;Vivas A;Johansson S;van der Meer A;Tenje M;Maegdefessel L
器官芯片（OoC）的发展彻底改变了体外细胞培养实验，可以更好地模拟人类生理学和病理生理学，从而使研究人员能够对疾病机制获得更有意义的见解。最近，一些芯片上的心脏和芯片上的血管模型已被证明可以概括人类心血管系统的基本方面。这些 2D 和 3D 系统包括具有分层结构的芯片上心脏和芯片上血管中同步跳动的心肌细胞，并包含生理和病理剪切应力条件。由于利用患者来源的细胞及其微环境的精确控制，发现新靶点和使用基于芯片的平台进行药物测试的机会大大增加。这些器官模型将为未来个性化心血管医学和改善患者护理的方法提供重要资产。然而，某些技术和生物学挑战仍然存在，使得全球利用 OoC 来解决心血管科学中尚未解答的问题仍然相当具有挑战性。这篇综述文章旨在介绍和总结已发表的心脏和血管芯片方面的工作，同时也提供关于如何使用这些先进的体外系统根据患者特定特征定制疾病模型的展望和观点。
The development of organs-on-chip (OoC) has revolutionized in vitro cell-culture experiments by allowing a better mimicry of human physiology and pathophysiology that has consequently led researchers to gain more meaningful insights into disease mechanisms. Several models of hearts-on-chips and vessels-on-chips have been demonstrated to recapitulate fundamental aspects of the human cardiovascular system in the recent past. These 2D and 3D systems include synchronized beating cardiomyocytes in hearts-on-chips and vessels-on-chips with layer-based structures and the inclusion of physiological and pathological shear stress conditions. The opportunities to discover novel targets and to perform drug testing with chip-based platforms have substantially enhanced, thanks to the utilization of patient-derived cells and precise control of their microenvironment. These organ models will provide an important asset for future approaches to personalized cardiovascular medicine and improved patient care. However, certain technical and biological challenges remain, making the global utilization of OoCs to tackle unanswered questions in cardiovascular science still rather challenging. This review article aims to introduce and summarize published work on hearts- and vessels-on chips but also to provide an outlook and perspective on how these advanced in vitro systems can be used to tailor disease models with patient-specific characteristics.
登录
查看更多内容
影响因子：
6.1
作者：
de Mello, Camilly P. Fires;Carmona-Moran, Carlos;Hickman, James J.
通讯作者：
Hickman, James J.
影响因子：
3.2
作者：
Ellis, Bradley W.;Acun, Aylin;Zorlutuna, Pinar
通讯作者：
Zorlutuna, Pinar
DOI：
10.4137/aci.s28905
发表时间：
2015
期刊：
Analytical chemistry insights
影响因子：
--
作者：
An F;Qu Y;Liu X;Zhong R;Luo Y
通讯作者：
Luo Y
影响因子：
6.1
作者：
Agarwal A;Goss JA;Cho A;McCain ML;Parker KK
通讯作者：
Parker KK
影响因子：
21.3
作者：
Artegiani, Benedetta;Hendriks, Delilah;Clevers, Hans
通讯作者：
Clevers, Hans