Maternal immunization with pneumococcal surface protein A protects against pneumococcal infections among derived offspring.
Maternal immunization with pneumococcal surface protein A protects against pneumococcal infections among derived offspring.
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DOI：
10.1371/journal.pone.0027102
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Yamanaka N
中科院分区：
文献类型：
--
作者：
Kono M;Hotomi M;Hollingshead SK;Briles DE;Yamanaka N
病原体特异性抗体在预防肺炎球菌携带和感染方面发挥着重要作用。然而，新生儿和婴儿表现出先天性和适应性免疫反应受损，这导致他们对肺炎球菌高度敏感。为了保护新生儿和婴儿免受肺炎球菌感染，在很小的时候就引发特异性的保护性免疫反应非常重要。在这项研究中，我们研究了肺炎球菌表面蛋白 A (PspA) 母亲免疫接种对肺炎球菌携带、肺炎和败血症的保护性免疫力。母鼠在交配前用重组 PspA (rPspA) 和霍乱毒素 B 亚基 (CTB) 进行鼻内免疫。抗 PspA 特异性 IgG（主要是 IgG1）在免疫母体的血清和乳汁及其幼崽的血清中以高水平存在。在经 PspA 免疫的母亲产下和/或母乳喂养的幼崽中，经清洗的鼻组织和肺匀浆中的肺炎球菌密度显着降低。通过胎盘或乳汁接受抗 PspA 抗体的幼犬，在遭受各种类型肺炎球菌致命性全身感染后，其存活率显着延长。目前的研究结果强烈表明，母亲接种 PspA 疫苗是预防儿童早期肺炎球菌感染的一种有吸引力的策略。 （191字）
Pathogen-specific antibody plays an important role in protection against pneumococcal carriage and infections. However, neonates and infants exhibit impaired innate and adaptive immune responses, which result in their high susceptibility to pneumococci. To protect neonates and infants against pneumococcal infection it is important to elicit specific protective immune responses at very young ages. In this study, we investigated the protective immunity against pneumococcal carriage, pneumonia, and sepsis induced by maternal immunization with pneumococcal surface protein A (PspA). Mother mice were intranasally immunized with recombinant PspA (rPspA) and cholera toxin B subunit (CTB) prior to being mated. Anti-PspA specific IgG, predominantly IgG1, was present at a high level in the serum and milk of immunized mothers and in the sera of their pups. The pneumococcal densities in washed nasal tissues and in lung homogenate were significantly reduced in pups delivered from and/or breast-fed by PspA-immunized mothers. Survival after fatal systemic infections with various types of pneumococci was significantly extended in the pups, which had received anti-PspA antibody via the placenta or through their milk. The current findings strongly suggest that maternal immunization with PspA is an attractive strategy against pneumococcal infections during early childhood. (191 words)
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影响因子：
3.1
作者：
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