Research Techniques Made Simple: High-Throughput Sequencing of the T-Cell Receptor.
Research Techniques Made Simple: High-Throughput Sequencing of the T-Cell Receptor.
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DOI：
10.1016/j.jid.2017.04.001
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发表时间：
2017-06
期刊：
影响因子：
--
通讯作者：
Teunissen MBM
中科院分区：
文献类型：
--
作者：
Matos TR;de Rie MA;Teunissen MBM
近年来，T细胞受体（TCR）的高通量测序（HTS）发展迅速，为鉴定和定量任何生物样品中存在的每种不同T细胞克隆提供了独特的机会。这种灵敏和准确的技术提供了完整T细胞库中每个单独克隆的相对频率，允许研究特定的单个感兴趣的T细胞克隆以及整个群体的所有不同克隆的伴随分析。HTS对于扩大我们对TCR库在稳态或病理条件下的多样性的知识，以及理解抗原特异性T细胞应答的发展和动力学至关重要，这些抗原特异性T细胞应答决定了保护性免疫（即疫苗接种）和免疫相关疾病（即自身免疫和癌症）。有趣的是，HTS可以针对个性化医疗进行定制，有可能监测治疗干预并揭示预后和诊断生物标志物。本文就HTS技术的方法学、进展和局限性作一简要综述，并介绍HTS技术在皮肤病学研究领域的应用，包括研究T细胞在过敏性皮炎中的发病机制及其在皮肤T细胞淋巴瘤诊断、早期复发检测和治疗监测中的应用。
High-throughput sequencing (HTS) of the T-cell receptor (TCR) has advanced rapidly in recent years, giving the unique opportunity to identify and quantify every distinct T-cell clone present within any biological sample. This sensitive and accurate technique provides the relative frequency of each individual clone within the full T-cell repertoire, allowing to study particular single T-cell clones of interest as well as the concomitant analysis of all different clones of the whole population. HTS is essential to expand our knowledge on the diversity of the TCR repertoire in homeostasis or pathologic conditions, as well as to understand the development and kinetics of antigen-specific T-cell responses that critically determine protective immunity (i.e. vaccination) and immune-related disorders (i.e. auto-immunity and cancer). Interestingly, HTS can be tailored for personalized medicine, having the potential to monitor therapeutic interventions and reveal prognostic and diagnostic biomarkers. In this article, we briefly review the methodology, advances and limitations of HTS, and describe emerging applications of this novel technique in the field of investigative dermatology, including studying the pathogenesis of T cells in allergic dermatitis and its application in diagnosis, early recurrence detection and therapy monitoring of cutaneous T-cell lymphoma.
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