Baculovirus Vectors
Baculovirus Vectors
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杆状病毒载体
DOI：
10.2165/00129785-200303010-00007
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
C. Hofmann
中科院分区：
文献类型：
--
作者：
A. Hüser;C. Hofmann
已经开发了各种方法来在哺乳动物细胞中转移和表达基因。每种方法，无论是病毒性的、非病毒性的还是基于物理的，都具有独特的有利特征，但也有关于满足期望的和特定的需求的缺点。自1983年以来，杆状病毒已被用于在其天然宿主（昆虫）细胞中表达由强昆虫病毒启动子控制的重组基因。1995年首次报道重组杆状病毒能够将基因导入哺乳动物细胞。这些基因被表达，条件是它们由在哺乳动物细胞中有活性的启动子控制。从那时起，已经开发了各种载体变体，并描述了许多潜在的和有意义的应用。杆状病毒载体作为哺乳动物细胞基因传递载体的应用不断增加，并且该系统正在不断改进，这并不奇怪，基于该系统将基因转移到哺乳动物细胞中的便利性，杆状病毒可以应用于基于细胞的药物筛选测定中，以克服产生稳定细胞系所需的长时间。杆状病毒载体能够将非常大的DNA序列递送到哺乳动物细胞中，并且还可以产生用于毒性基因产物的载体。此外，在没有合适的细胞培养系统的情况下，杆状病毒是启动病毒感染的有价值的工具。此外，最近的研究表明，载体可以在体内应用。根据研究的设计，杆状病毒载体允许持续的基因表达或能够诱导针对递送和/或展示的基因产物的免疫应答。后者提供了产生针对某些蛋白质的单克隆抗体的机会，这些蛋白质通过其他方法已经失败。此外，它指出了杆状病毒载体作为新型疫苗的潜在用途。因此，杆状病毒载体被认为是各种产品机会的使能技术。
Various methods have been developed to transfer and express genes in mammalian cells. Each method, whether virally, non-virally, or physically-based, has unique favorable features, but also drawbacks with respect to meeting desired and specific needs. Baculoviruses have been used since 1983 to express recombinant genes controlled by strong insect-virus promoters in their natural host (insect) cells. Today this is a well-established and easy to handle system for producing large quantities of recombinant proteins for numerous purposes.In 1995 it was first published that recombinant baculoviruses are able to deliver genes into mammalian cells. These genes are expressed provided that they are controlled by a promoter which is active in mammalian cells. Since then, various vector variants have been developed and numerous potential and meaningful applications have been described. It is not surprising that the use of baculovirus vectors as mammalian cell gene delivery vectors is constantly increasing and that the system is undergoing permanent improvements.Based on the convenience of the system to transfer genes into mammalian cells, baculoviruses can be applied in cell-based assays for drug screening to overcome the long periods of time required to generate stable cell lines. Baculovirus vectors are able to deliver very large DNA sequences into mammalian cells and vectors for toxic gene products can also be generated. In addition, baculoviruses are valuable tools for launching viral infection in cases where there is no appropriate cell culture system. Moreover, recent research has shown that the vectors can be applied in vivo. Depending on the design of the study, baculovirus vectors allow for sustained gene expression or are able to induce an immune response directed against the delivered and/or displayed gene product. The latter offers the opportunity to generate monoclonal antibodies against certain proteins that have failed by other means. In addition, it points to the potential usefulness of baculovirus vectors as new kinds of vaccines. Baculovirus vectors are therefore considered an enabling technology for various product opportunities.
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重组杆状病毒介导的基因转移至小鼠眼中。
影响因子：
4.4
作者：
Haeseleer,F;Imanishi,Y;Saperstein,DA;Palczewski,K
通讯作者：
Palczewski,K
影响因子：
7.7
作者：
Ma, L;Tamarina, N;Philipson, LH
通讯作者：
Philipson, LH
DOI：
10.1073/pnas.97.20.11002
发表时间：
2000-09
影响因子：
11.1
作者：
G. Ye;B. Roizman
通讯作者：
G. Ye;B. Roizman
DOI：
10.1073/pnas.93.6.2348
发表时间：
1996-03-19
影响因子：
11.1
作者：
Boyce, FM;Bucher, NLR
通讯作者：
Bucher, NLR
杆状病毒：在昆虫细胞中高水平表达。
影响因子：
4
作者：
Miller,LK
通讯作者：
Miller,LK