Establishment and a comparative transcriptomic analysis of a male-specific cell line from the African malaria mosquito Anopheles gambiae.
Establishment and a comparative transcriptomic analysis of a male-specific cell line from the African malaria mosquito Anopheles gambiae.
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DOI：
10.1038/s41598-022-10686-y
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发表时间：
2022-04-27
影响因子：
4.6
通讯作者：
Krzywinski, Jaroslaw
中科院分区：
文献类型：
--
作者：
Krzywinska, Elzbieta;Ferretti, Luca;Krzywinski, Jaroslaw
细胞系允许研究在整个生物体中可能不容易处理的各种生物过程。在这里，我们已经建立了第一个男性特异性细胞系从非洲疟疾蚊子，冈比亚按蚊。这些细胞命名为AgMM，来源于性别分选的新生幼虫，在建立后能够进行多次自发收缩，表明它们的成肌细胞来源。将它们的转录组与A.冈比亚来源的Sua5.1血细胞揭示了每种细胞系的不同分子特征，包括许多在AgMM细胞中高度和独特表达的肌肉相关基因。此外，AgMM细胞表达初级性别决定基因Yob，并支持雄性性别决定和剂量补偿途径。因此，AgMM细胞系代表了这些男性特异性过程的分子和生物化学体外研究的有价值的工具。在更广泛的背景下，本研究中产生的丰富的转录组学数据集有助于更好地理解A.冈比亚的基因组，揭示了这两种细胞类型的生物学，促进其预期用于各种基于细胞的测定。
Cell lines allow studying various biological processes that may not be easily tractable in whole organisms. Here, we have established the first male-specific cell line from the African malaria mosquito, Anopheles gambiae. The cells, named AgMM and derived from the sex-sorted neonate larvae, were able to undergo spontaneous contractions for a number of passages following establishment, indicating their myoblast origin. Comparison of their transcriptome to the transcriptome of an A. gambiae-derived Sua5.1 hemocyte cells revealed distinguishing molecular signatures of each cell line, including numerous muscle-related genes that were highly and uniquely expressed in the AgMM cells. Moreover, the AgMM cells express the primary sex determiner gene Yob and support male sex determination and dosage compensation pathways. Therefore, the AgMM cell line represents a valuable tool for molecular and biochemical in vitro studies of these male-specific processes. In a broader context, a rich transcriptomic data set generated in this study contributes to a better understanding of transcribed regions of the A. gambiae genome and sheds light on the biology of both cell types, facilitating their anticipated use for various cell-based assays.
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影响因子：
6.7
作者：
Lombardo F;Ghani Y;Kafatos FC;Christophides GK
通讯作者：
Christophides GK
DOI：
10.1073/pnas.2114242118
发表时间：
2021-11-02
影响因子：
11.1
作者：
Gomes, Fabio M.;Tyner, Miles D. W.;Barillas-Mury, Carolina
通讯作者：
Barillas-Mury, Carolina
影响因子：
6.7
作者：
Kambris Z;Blagborough AM;Pinto SB;Blagrove MS;Godfray HC;Sinden RE;Sinkins SP
通讯作者：
Sinkins SP
影响因子：
12.3
作者：
Lee H;McManus CJ;Cho DY;Eaton M;Renda F;Somma MP;Cherbas L;May G;Powell S;Zhang D;Zhan L;Resch A;Andrews J;Celniker SE;Cherbas P;Przytycka TM;Gatti M;Oliver B;Graveley B;MacAlpine D
通讯作者：
MacAlpine D
影响因子：
5.8
作者：
Barletta, Ana Beatriz F.;Trisnadi, Nathanie;Barillas-Mury, Carolina
通讯作者：
Barillas-Mury, Carolina