Abscopal Activation of Microglia in Embryonic Fish Brain Following Targeted Irradiation with Heavy-Ion Microbeam.
Abscopal Activation of Microglia in Embryonic Fish Brain Following Targeted Irradiation with Heavy-Ion Microbeam.
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DOI：
10.3390/ijms18071428
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发表时间：
2017-07-04
影响因子：
5.6
通讯作者：
Oda S
中科院分区：
文献类型：
--
作者：
Yasuda T;Kamahori M;Nagata K;Watanabe-Asaka T;Suzuki M;Funayama T;Mitani H;Oda S
当脊椎动物中枢神经系统受损时，小胶质细胞通过吞噬作用清除凋亡细胞。电离辐射 (IR) 诱导青鳉 (Oryzias latipes) 胚胎中脑细胞凋亡和小胶质细胞活化，其中载脂蛋白 E (ApoE) 在小胶质细胞激活的后期上调。在这项研究中，我们发现另一种小胶质细胞标志物 l-plastin（淋巴细胞胞质蛋白 1）在 IR 诱导的吞噬作用的初始阶段上调，此时激活的小胶质细胞改变了其形态并增加了吞噬作用。迁移的动力。我们进一步使用碳离子准直微束（直径 250 μm）对胚胎中脑进行靶向照射，发现仅在位于照射区域的小胶质细胞中诱导 l-plastin 上调。然后，激活的小胶质细胞可能会迁移到辐射区域之外，并在辐射后持续超过 3 天的时间扩散到胚胎大脑，表达 ApoE 并具有激活的形态。这些发现表明，l-plastin 和 ApoE 可以分别是青鳉胚胎脑中初始和后期激活的小胶质细胞的生物标志物，并且红外辐射引起的小胶质细胞的远隔和持续激活可能是辐射后远隔和/或不良反应的原因。
Microglia remove apoptotic cells by phagocytosis when the central nervous system is injured in vertebrates. Ionizing irradiation (IR) induces apoptosis and microglial activation in embryonic midbrain of medaka (Oryzias latipes), where apolipoprotein E (ApoE) is upregulated in the later phase of activation of microglia In this study, we found that another microglial marker, l-plastin (lymphocyte cytosolic protein 1), was upregulated at the initial phase of the IR-induced phagocytosis when activated microglia changed their morphology and increased motility to migrate. We further conducted targeted irradiation to the embryonic midbrain using a collimated microbeam of carbon ions (250 μm diameter) and found that the l-plastin upregulation was induced only in the microglia located in the irradiated area. Then, the activated microglia might migrate outside of the irradiated area and spread through over the embryonic brain, expressing ApoE and with activated morphology, for longer than 3 days after the irradiation. These findings suggest that l-plastin and ApoE can be the biomarkers of the activated microglia in the initial and later phase, respectively, in the medaka embryonic brain and that the abscopal and persisted activation of microglia by IR irradiation could be a cause of the abscopal and/or adverse effects following irradiation.
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影响因子：
4.6
作者：
Acharya MM;Green KN;Allen BD;Najafi AR;Syage A;Minasyan H;Le MT;Kawashita T;Giedzinski E;Parihar VK;West BL;Baulch JE;Limoli CL
通讯作者：
Limoli CL
DOI：
10.1016/j.bbrc.2012.03.139
发表时间：
2012-05-04
影响因子：
3.1
作者：
Huang T;Cui J;Li L;Hitchcock PF;Li Y
通讯作者：
Li Y
DOI：
10.1158/1078-0432.ccr-11-2903
发表时间：
2013-05-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Greene-Schloesser D;Moore E;Robbins ME
通讯作者：
Robbins ME
影响因子：
6.2
作者：
Eyo, Ukpong;Dailey, Michael E.
通讯作者：
Dailey, Michael E.
影响因子：
6.2
作者：
Baumgart, Emily Violette;Barbosa, Joana S.;Ninkovic, Jovica
通讯作者：
Ninkovic, Jovica