Building protein networks in synthetic systems from the bottom-up.
Building protein networks in synthetic systems from the bottom-up.
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DOI：
10.1016/j.biotechadv.2021.107753
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发表时间：
2021-07
影响因子：
16
通讯作者：
中科院分区：
文献类型：
--
作者：
合成生物学的最新发展扩大了自下而上在活细胞外设计和构建蛋白质网络的能力。新的能力使我们能够组装蛋白质网络，用于细胞通路的基础研究，细胞外蛋白质的表达和构建组织材料。此外，天然和合成蛋白质网络的整合使合成或人工细胞的新功能成为可能。在这里，我们回顾了自下而上在脂质体、油包水液滴和生物材料中组装蛋白质网络的基础技术。我们涵盖了蛋白质网络在生物转导途径，能量自我供应系统，细胞环境传感器和无细胞蛋白质支架中的最新应用。我们还回顾了组装蛋白质网络的新技术，包括多蛋白纯化方法，高通量检测筛选平台和脂质体的可控融合。最后，我们提出了构建蛋白质网络的现有挑战，这些网络的复杂性和动态响应与自然系统类似。本文综述了我们对合成和天然蛋白质网络的理解中存在的差距。它提出了为各种生物医学应用开发智能和弹性蛋白质网络的愿景。
The recent development of synthetic biology has expanded the capability to design and construct protein networks outside of living cells from the bottom-up. The new capability has enabled us to assemble protein networks for the basic study of cellular pathways, expression of proteins outside cells, and building tissue materials. Furthermore, the integration of natural and synthetic protein networks has enabled new functions of synthetic or artificial cells. Here, we review the underlying technologies for assembling protein networks in liposomes, water-in-oil droplets, and biomaterials from the bottom-up. We cover the recent applications of protein networks in biological transduction pathways, energy self-supplying systems, cellular environmental sensors, and cell-free protein scaffolds. We also review new technologies for assembling protein networks, including multiprotein purification methods, high-throughput assay screen platforms, and controllable fusion of liposomes. Finally, we present existing challenges towards building protein networks that rival the complexity and dynamic response akin to natural systems. This review addresses the gap in our understanding of synthetic and natural protein networks. It presents a vision towards developing smart and resilient protein networks for various biomedical applications.
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