Label-free quantitative mass spectrometry reveals a panel of differentially expressed proteins in colorectal cancer.
Label-free quantitative mass spectrometry reveals a panel of differentially expressed proteins in colorectal cancer.
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DOI：
10.1155/2015/365068
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发表时间：
2015
影响因子：
--
通讯作者：
Gao CF
中科院分区：
文献类型：
--
作者：
Fan NJ;Gao JL;Liu Y;Song W;Zhang ZY;Gao CF
为了鉴定CRC中涉及的潜在生物标志物，应用鸟枪蛋白质组学方法，使用高效液相色谱和质谱法鉴定三种CRC和匹配的正常粘膜组织中的可溶性蛋白。然后对三种CRC和匹配的正常粘膜组织进行无标记蛋白质谱分析以定量和比较蛋白质。结果显示，在784个鉴定的蛋白质中，有67个与CRC相关（28个上调，39个下调）。通过基因本体论和大卫数据库对与细胞结合、细胞结构、信号转导、细胞粘附等生物学过程相关的差异蛋白质进行定位和功能分析，筛选出原肌球蛋白3（tropomyosin-3，TPM 3）、内质网驻留蛋白29（endoplasmic reticulum resident protein 29，ERp 29）、18 kDa阳离子抗菌蛋白（cationic antimicrobial protein，CAMP）、Western blot分析证实热休克蛋白8（HSPA 8）在结直肠癌组织和7种细胞系中表达上调。免疫组化法检测69例CRC中TPM 3、ERp 29、CAMP和HSPA 8的表达。TPM 3、ERp 29、CAMP和HSPA 8的组合可以从匹配的正常粘膜中识别CRC，使用IHC评分实现73.2%的准确性。这些结果表明，TPM 3、ERp 29、CAMP和HSPA 8是CRC的非常有潜力的IHC诊断生物标志物。
To identify potential biomarkers involved in CRC, a shotgun proteomic method was applied to identify soluble proteins in three CRCs and matched normal mucosal tissues using high-performance liquid chromatography and mass spectrometry. Label-free protein profiling of three CRCs and matched normal mucosal tissues were then conducted to quantify and compare proteins. Results showed that 67 of the 784 identified proteins were linked to CRC (28 upregulated and 39 downregulated). Gene Ontology and DAVID databases were searched to identify the location and function of differential proteins that were related to the biological processes of binding, cell structure, signal transduction, cell adhesion, and so on. Among the differentially expressed proteins, tropomyosin-3 (TPM3), endoplasmic reticulum resident protein 29 (ERp29), 18 kDa cationic antimicrobial protein (CAMP), and heat shock 70 kDa protein 8 (HSPA8) were verified to be upregulated in CRC tissue and seven cell lines through western blot analysis. Furthermore, the upregulation of TPM3, ERp29, CAMP, and HSPA8 was validated in 69 CRCs byimmunohistochemistry (IHC) analysis. Combination of TPM3, ERp29, CAMP, and HSPA8 can identify CRC from matched normal mucosal achieving an accuracy of 73.2% using IHC score. These results suggest that TPM3, ERp29, CAMP, and HSPA8 are great potential IHC diagnostic biomarkers for CRC.
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影响因子：
2.6
作者：
Fan NJ;Gao CF;Zhao G;Wang XL;Liu QY
通讯作者：
Liu QY
癌组学对癌症生物标志物发现的贡献。
影响因子：
37.3
作者：
Cho, William C S
通讯作者：
Cho, William C S
影响因子：
4.8
作者：
Gombart, AF;Borregaard, N;Koeffler, HP
通讯作者：
Koeffler, HP
影响因子：
2.7
作者：
Chaffer, Christine L.;Thompson, Erik W.;Williams, Elizabeth D.
通讯作者：
Williams, Elizabeth D.
影响因子：
3.8
作者：
Choi HS;Yim SH;Xu HD;Jung SH;Shin SH;Hu HJ;Jung CK;Choi JY;Chung YJ
通讯作者：
Chung YJ