Meiotic drive does not impede success in sperm competition in the stalk-eyed fly, Teleopsis dalmanni
Meiotic drive does not impede success in sperm competition in the stalk-eyed fly, Teleopsis dalmanni
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减数分裂驱动并不妨碍茎眼蝇（Teleopsis dalmanni）精子竞争的成功
DOI：
10.1101/2022.09.09.507334
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Bates S
中科院分区：
文献类型：
--
作者：
Bates S
在男性X连锁的减数分裂驱动系统中，司机会导致携带Y的精子退化，导致女性偏向后代性别比例。这潜在地导致了驱动染色体的双重传播优势。然而，携带驱动力的精子在精子竞争中往往表现不佳，限制了它们的传播能力。我们使用柄眼蝇(Teleopsis Dalmanni)来研究X-连锁性别比(SR)减数分裂驱动系统的成功。在这个物种中，一只雌性与多只雄性交配的多雄交配很常见。最近的发现表明，SR雄性在交配过程中转移的可存活精子数量与野生型(ST)雄性相同，这意味着它们在精子竞争下不一定会降低生育能力。进行了相互交配试验，以衡量SR和ST精子在双交配雌性中的成功率，其中SR或ST雄性首先交配，然后是另一种基因的雄性。SR和ST雄蜂所产后代数无显著差异。无论雄蜂是第一次交配还是第二次交配，这种等价性都是成立的。我们表明这些结果与之前的研究一致，这些研究表明SR男性精子在精子竞争中表现不佳。未来的实验将确定雄性SR是否在更高的压力条件下保持竞争能力，这种压力很可能在自然界中经历，在这种情况下，雌性与多个雄性重复交配。目前的研究结果有助于解释该物种野生种群中约20%的减数分裂驱动频率。影响摘要减数分裂驱动基因是自私的遗传元件，扭曲孟德尔遗传模式，使传递偏向于它们。我们使用柄眼苍蝇，Teleopsis dalmanni，来研究与减数分裂驱动基因相关的适合度效应，该基因被称为性别比(SR)，它与X染色体相连。在雄性中，SR会破坏带有Y的精子，这意味着只有带有X的精子才能存活，而与雄性交配的雌性会产生全是雌性的后代。这为SR基因提供了双重的传播优势，因为它会传播给所有的后代。我们最近发现，驱动力雄性已经进化出补偿机制来应对减数分裂驱动导致的精子破坏。它们的睾丸大大增大，从而能够产生更多的精子。当驱使雄性与雌性交配时，它们产生的精子和后代与野生型雄性一样多。基于这一发现，我们测量了在精子竞争中，驱动男性精子与来自非携带者男性的精子的表现-来自不同男性的精子竞争受精卵子。进行了两次交配试验，其中一只雌性与一只驱动器交配一次，一次与非携带者雄性交配。通过对后代进行基因分型，我们发现驱逐型雄性与非携带型竞争种所生后代的数量没有差异，这一结果与其他物种的结果进行了对比。通常情况下，Drive雄性在精子竞争中表现不佳，他们的传播受到精子竞争的严重限制。在柄眼苍蝇中，雌性与许多雄性多次交配是常态，但这似乎并不抑制驱使雄性的生育能力。精子竞争下的驱动力的成功有助于解释为什么在自然种群中，驱动力的频率通常在20%左右。达尔曼尼。
In male X-linked meiotic drive systems, the driver causes degeneration of Y-bearing sperm, leading to female-biased offspring sex ratios. This potentially leads to a two-fold transmission advantage to drive chromosomes. However, drive-bearing sperm often do poorly in sperm competition, limiting their ability to spread. We use the stalk-eyed fly,Teleopsis dalmanni, to investigate the success of the X-linkedSex Ratio(SR) meiotic drive system. In this species, polyandrous matings, where a female mates with multiple males, are common. Recent findings demonstrate SR males transfer the same numbers of viable sperm as wildtype (ST) males during mating, implying that they do not necessarily have reduced fertility under sperm competition. Reciprocal mating trials were performed to measure the success of SR and ST sperm in double mated females, with either a SR or ST male mated first followed by a male of the alternative genotype. There was no significant difference in the number of offspring sired by SR and ST males. This equivalence held regardless of whether the SR male mated first or second. We show these results are consistent with previous studies that suggested SR male sperm do poorly in sperm competition. Future experiments will determine whether the competitive ability of SR males is maintained under higher stress conditions likely to be experienced in nature, in which females mate repeatedly with multiple males. The results from the current study helps to explain the high meiotic drive frequency of around 20% in wild populations in this species.Impact SummaryMeiotic drive genes are selfish genetic elements that distort Mendelian patterns of inheritance to bias transmission in their favour. We use the stalk-eyed fly,Teleopsis dalmanni, to investigate the fitness effects associated with a meiotic drive gene calledSex Ratio(SR), which is linked to the X chromosome. In males, SR destroys Y-bearing sperm, meaning only X-bearing sperm are viable, and females who mate with drive males sire all-female broods. This confers a two-fold transmission advantage to the SR gene, as it is transmitted to all offspring.We recently discovered that drive males have evolved compensatory mechanisms to cope with the sperm destruction caused by meiotic drive. They have greatly enlarged testes, allowing them to produce more sperm. When drive males mate with females, they deliver as many sperm and sire as many offspring as wildtype males. Building on this finding, we measured how drive male sperm performs against sperm from a non-carrier male in sperm competition – where the sperm from different males compete to fertilise an egg. Double mating trials were performed, where a single female was mated once to a drive and once to a non-carrier male. By genotyping offspring, we show that the number of offspring sired by the drive male was not different from the number sired by the non-carrier competitor.These findings contrast with those in other species. Typically, drive males do poorly in sperm competition and their spread is severely restricted by sperm competition. In stalk-eyed flies, female multiple mating with many males is the norm, but this does not appear to inhibit the fertility of drive males. The success of drive under sperm competition helps to explain the high frequency of drive around 20% in natural populations ofT. dalmanni.
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X染色体驱动对茎眼果蝇Cyrtodiopsis dalmanni的适应性影响
影响因子：
2.1
作者：
G. Wilkinson;P. Johns;E. Kelleher;Mario L. Muscedere;A. Lorsong
通讯作者：
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茎眼果蝇的雄性眼跨度通过减数分裂驱动抑制表明遗传质量
影响因子：
64.8
作者：
G. Wilkinson;Daven C. Presgraves;Lili Crymes
通讯作者：
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T.Kumamaru；H.Satoh；T.Omura；M.Okawa：遗传。
DOI：
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发表时间：
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期刊：
影响因子：
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作者：
通讯作者：
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男性性装饰的大小与生殖形态呈正相关，并在茎眼射telepopsis dalmanni中增强了生育能力。
影响因子：
3.4
作者：
Rogers, David W.;Denniff, Matthew;Chapman, Tracey;Fowler, Kevin;Pomiankowski, Andrew
通讯作者：
Pomiankowski, Andrew
影响因子：
4.5
作者：
Reinhardt JA;Brand CL;Paczolt KA;Johns PM;Baker RH;Wilkinson GS
通讯作者：
Wilkinson GS