Expression of Interleukin-26 is upregulated in inflammatory bowel disease.
Expression of Interleukin-26 is upregulated in inflammatory bowel disease.
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DOI：
10.3748/wjg.v23.i30.5519
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发表时间：
2017-08-14
影响因子：
4.3
通讯作者：
Andoh A
中科院分区：
文献类型：
--
作者：
Fujii M;Nishida A;Imaeda H;Ohno M;Nishino K;Sakai S;Inatomi O;Bamba S;Kawahara M;Shimizu T;Andoh A
目的：探讨炎症性肠病(IBD)患者炎症黏膜中白介素26(IL-26)的表达及其功能。从手术切除的结肠组织中分离出人结肠上皮下肌成纤维细胞(SEMF)。免疫组织化学方法检测IL-26蛋白及其受体复合体的表达。实时定量聚合酶链式反应检测IL-26诱导的基因表达。免疫印迹法和特异性小干扰(Si)RNA转染法检测细胞内信号转导途径。IBD患者炎症黏膜中IL-26的mRNA和蛋白表达明显增强。IL-26受体复合体在体内和体外均可在结肠SEMF中表达。IL-26刺激结肠间充质干细胞IL-6和IL-8的mRNA表达。丝裂原活化蛋白激酶和磷脂酰肌醇3-激酶的抑制剂、信号转导和转录激活因子1/3的siRNAs、核因子-kappaB和激活蛋白-1显著降低IL-26诱导的IL-6和IL-8的mRNA表达。提示IL-26通过诱导炎性介质参与IBD的病理生理过程。
To investigate interleukin (IL)-26 expression in the inflamed mucosa of patients with inflammatory bowel disease (IBD) and the function of IL-26. Human colonic subepithelial myofibroblasts (SEMFs) were isolated from colon tissue surgically resected. The expression of IL-26 protein and its receptor complex was analyzed by immunohistochemistry. The gene expression induced by IL-26 was evaluated by real-time polymerase chain reaction. Intracellular signaling pathways were evaluated by immunoblotting and specific small interfering (si) RNA transfection. The mRNA and protein expression of IL-26 were significantly enhanced in the inflamed mucosa of patients with IBD. IL-26 receptor complex was expressed in colonic SEMFs in vivo and in vitro. IL-26 stimulated the mRNA expression of IL-6 and IL-8 in colonic SEMFs. The inhibitors of mitogen-activated protein kinases and phosphoinositide 3-kinase, and siRNAs for signal transducers and activator of transcription 1/3, nuclear factor-kappa B and activator protein-1 significantly reduced the mRNA expression of IL-6 and IL-8 induced by IL-26. These results suggest that IL-26 plays a role in the pathophysiology of IBD through induction of inflammatory mediators.
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影响因子：
64.8
作者：
Cella, Marina;Fuchs, Anja;Vermi, William;Facchetti, Fabio;Otero, Karel;Lennerz, Jochen K. M.;Doherty, Jason M.;Mills, Jason C.;Colonna, Marco
通讯作者：
Colonna, Marco
影响因子：
30.5
作者：
Manel, Nicolas;Unutmaz, Derya;Littman, Dan R.
通讯作者：
Littman, Dan R.
影响因子：
4.4
作者：
Andoh, Akira;Shioya, Makoto;Fujiyama, Yoshihide
通讯作者：
Fujiyama, Yoshihide
影响因子：
4.4
作者：
Pene, Jerome;Chevalier, Sylvie;Gascan, Hugues
通讯作者：
Gascan, Hugues
影响因子：
20.3
作者：
Hughes, Tiffany;Becknell, Brian;Caligiuri, Michael A.
通讯作者：
Caligiuri, Michael A.