A role for GATA5 in Xenopus endoderm specification.
A role for GATA5 in Xenopus endoderm specification.
复制标题
GATA5 在非洲爪蟾内胚层规范中的作用。
作者：
H. Weber;C. Symes;M. Walmsley;A. Rodaway;R. Patient
内胚层产生肠和作为肠管的副产物发育的组织，包括肺、肝和胰腺。在这里，我们表明，GATA 5，锌指转录因子，表达在富含蛋黄的植物细胞的爪蟾胚胎从早期原肠胚阶段向前，当这些细胞成为致力于形成内胚层。在原肠胚中期，GATA 5仅限于囊胚口下内胚层，并且是迄今为止发现的该内胚层细胞亚群的第一个分子标记物。我们表明，GATA 4和GATA 5是有效的诱导剂的内胚层标记基因在动物帽检测，而其他加塔因素诱导这些基因只有微弱的，如果在所有。当注射到背侧边缘区，GATA 5重新指定的前景中胚层走向内胚层的命运，从而破坏收敛和伸展运动通常经历的背侧中胚层。所得到的表型与注射FGF受体或T-box转录因子Xbra的显性阴性版本后观察到的表型非常相似，并且可以通过eFGF进行挽救。GATA 5将外胚层和中胚层细胞向内胚层重新指定的能力表明GATA 5在该胚层的形成中起重要作用。在动物帽测定中，GATA 5由高于已知诱导背中胚层和心脏的浓度的激活素以FGF非依赖性方式诱导。这些数据表明，内胚层诱导的新观点，即在没有FGF信号传导的情况下，由高水平的TGF-β指定，对于亚胚口内胚层特别正确。
The endoderm gives rise to the gut and tissues that develop as outgrowths of the gut tube, including the lungs, liver and pancreas. Here we show that GATA5, a zinc-finger transcription factor, is expressed in the yolk-rich vegetal cells of Xenopus embryos from the early gastrula stage onwards, when these cells become committed to form endoderm. At mid-gastrula stages, GATA5 is restricted to the sub-blastoporal endoderm and is the first molecular marker for this subset of endodermal cells so far identified. We show that GATA4 and GATA5 are potent inducers of endodermal marker genes in animal cap assays, while other GATA factors induce these genes only weakly, if at all. When injected into the dorsal marginal zone, GATA5 respecifies prospective mesoderm towards an endodermal fate, thereby disrupting the convergence and extension movements normally undergone by the dorsal mesoderm. The resulting phenotype is very similar to those seen after injection of dominant negative versions of the FGF-receptor or the T-box transcription factor, Xbra and can be rescued by eFGF. The ability of GATA5 to respecify ectodermal and mesodermal cells towards endoderm suggests an important role for GATA5 in the formation of this germlayer. In animal cap assays, GATA5 is induced by concentrations of activin above those known to induce dorsal mesoderm and heart, in an FGF-independent manner. These data indicate that the emerging view for endodermal induction in general, namely that it is specified by high levels of TGF-beta in the absence of FGF signalling, is specifically true for sub-blastoporal endoderm.
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影响因子：
4.6
作者：
M. Kofron;Teresa Demel;Jennifer B. Xanthos;J. Lohr;B. Sun;H. Sive;S. Osada;C. V. Wright;C. Wylie;J. Heasman
通讯作者：
M. Kofron;Teresa Demel;Jennifer B. Xanthos;J. Lohr;B. Sun;H. Sive;S. Osada;C. V. Wright;C. Wylie;J. Heasman
DOI：
10.1073/pnas.88.23.10642
发表时间：
1991-12
影响因子：
11.1
作者：
Leonard I. Zon;C. Mather;S. Burgess;M. E. Bolce;R. Harland;Stuart H. Orkin
通讯作者：
Leonard I. Zon;C. Mather;S. Burgess;M. E. Bolce;R. Harland;Stuart H. Orkin
影响因子：
4.6
作者：
O. Wessely;E. Robertis
通讯作者：
O. Wessely;E. Robertis
非洲爪蟾原肠胚形成过程中瓶细胞的行为和功能。
DOI：
10.1242/dev.103.1.211
发表时间：
1988
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Hardin,J;Keller,R
通讯作者：
Keller,R
影响因子：
10.5
作者：
Kuo, CT;Morrisey, EE;Leiden, JM
通讯作者：
Leiden, JM