Pre-Clinical Evaluation of a New Coral-Based Bone Scaffold
Pre-Clinical Evaluation of a New Coral-Based Bone Scaffold
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新型珊瑚骨支架的临床前评估
DOI：
10.1177/039463201402700209
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发表时间：
2014
影响因子：
3.5
通讯作者：
L. Muzio
中科院分区：
文献类型：
--
作者：
F. Carinci;A. Santarelli;L. Laino;F. Pezzetti;A. de Lillo;D. Parisi;F. Bambini;M. Procaccini;N. F. Testa;R. Cocchi;L. Muzio
珊瑚在世界范围内用于骨骼重建。使该材料适合植入的有利特性是（i）骨诱导，（ii）骨传导。这些特性已被证明在体内研究的动物模型和临床试验超过二十年的时间。聚（甲基丙烯酸2-羟乙酯）[聚（HEMA）]也是一种广泛使用的生物材料。通过使用珊瑚和聚（HEMA），最近已经合成了用于骨重建应用的支架。进行细胞学、组织学和遗传学分析以表征这种新的异质材料。分析了四种样品：（a）白色珊瑚（WC），（B）红珊瑚（RC），（c）WC加聚合物（WCP）和（d）RC加聚合物（RCP）。MTT法测定线粒体脱氢酶活性作为细胞毒性的间接检测方法。通过在兔胫骨中植入珊瑚和珊瑚基聚合物来揭示体内效应。4周后收集样品并进行组织学分析。为了评价细胞对珊瑚和珊瑚衍生的聚合物的遗传反应，将成骨细胞样细胞系（即MG 63）在含有（a）培养基、（B）培养基加珊瑚和（c）培养基加两种类型的支架（RCP或WCP）的威尔斯孔中培养。从细胞中提取的RNA被逆转录并在DNA 19.2K微阵列上杂交。在珊瑚和珊瑚基生物聚合物中没有检测到细胞毒性。组织学检查未发现炎症或不良反应。通过微阵列分析，154个克隆在RC和WC之间差异表达（81个上调，73个下调），而只有15个克隆被聚合物抑制。组织学评价不仅证实珊瑚是一种生物相容性材料，而且该聚合物没有不良反应。微阵列结果与细胞学和组织学分析一致，并提供了有关RC、WC和新聚合物遗传效应的进一步数据。
Coral is used worldwide for bone reconstruction. The favorable characteristics that make this material desirable for implantation are (i) osteoinduction, (ii) and osteoconduction. These proprieties have been demonstrated by in vivo studies with animal models and clinical trials over a twenty-year period. Also poly(2-hydroxyethylmethacrylate) [poly(HEMA)] is a widely used biomaterial. By using coral and poly(HEMA), a scaffold for bone reconstruction application has been recently synthesized. Cytological, histological and genetic analyses were performed to characterize this new alloplastic material. Four samples were analyzed: (a) white coral (WC), (b) red coral (RC), (c) WC plus polymer (WCP) and (d) RC plus polymer (RCP). Quantification of mitochondrial dehydrogenase activity by MTT assay was performed as indirect detector of cytotoxicity. In vivo effects were revealed by implanting corals and coral-based polymers in rabbit tibia. Samples were collected after 4 weeks and subjected to histological analysis. To evaluate the genetic response of cells to corals and coral-derived polymers an osteoblast-like cell line (i.e. MG63) was cultured in wells containing (a) medium, (b) medium plus corals and (c) medium plus two types of scaffolds (RCP or WCP). RNAs extracted from cells were retro-transcribed and hybridized on DNA 19.2K microarrays. No cytotoxicity was detected in corals and coral-based biopolymers. No inflammation or adverse effect was revealed by histological examination. By microarray analysis 154 clones were differentially expressed between RC and WC (81 up and 73 down regulated) whereas only 15 clones were repressed by the polymer. Histological evaluation not only confirmed that coral is a biocompatible material, but also that the polymer has no adverse effect. Microarray results were in agreement with cytological and histological analyses and provided further data regarding the genetic effects of RC, WC and the new polymer.
影响因子：
4.4
作者：
TABAS, JA;ZASLOFF, M;KAPLAN, FS
通讯作者：
KAPLAN, FS
影响因子：
4.6
作者：
J. Álvarez;P. Sohn;Xin Zeng;T. Doetschman;D. Robbins;R. Serra
通讯作者：
J. Álvarez;P. Sohn;Xin Zeng;T. Doetschman;D. Robbins;R. Serra
影响因子：
6.2
作者：
Atzet, Sarah;Curtin, Scott;Trinh, Phalen;Bryant, Stephanie;Ratner, Buddy
通讯作者：
Ratner, Buddy