Degradable poly(2-hydroxyethyl methacrylate)-co-polycaprolactone hydrogels for tissue engineering scaffolds.
Degradable poly(2-hydroxyethyl methacrylate)-co-polycaprolactone hydrogels for tissue engineering scaffolds.
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DOI：
10.1021/bm800686h
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发表时间：
2008-12
影响因子：
6.2
通讯作者：
Ratner, Buddy
中科院分区：
文献类型：
--
作者：
Atzet, Sarah;Curtin, Scott;Trinh, Phalen;Bryant, Stephanie;Ratner, Buddy
采用原子转移自由基聚合（ATRP）、可降解交联剂和大分子引发剂制备了用于组织工程构建的可生物降解聚甲基丙烯酸2-羟乙酯水凝胶。水凝胶由于其类似组织的机械顺应性和质量传递特性而成为组织工程支架的合适材料。然而，许多在医学上广泛应用的水凝胶是不可生物降解的，或者不能容易地从体内清除。选择聚（甲基丙烯酸2-羟乙酯）（pHEMA）作为支架材料，是因为其具有合理的机械强度、弹性、在医学中成功使用的悠久历史，并且因为其可以容易地制造成多种构型。在2kDa和50 kDa之间的各种分子量下研究pHEMA。适用于肾清除的分子量范围是实验设计中的重要因素。所制造的水凝胶含有聚己内酯（PCL）的低聚物嵌段作为交联剂，PCL是一种可水解和酶促降解的聚合物。此外，还使用还含有低聚PCL的可降解大分子引发剂来引发ATRP。链长、交联密度和聚合溶剂对pHEMA水凝胶的力学性能有很大影响。使用0.007 M NaOH、脂肪酶溶液和磷酸盐缓冲盐水表征pHEMA水凝胶的降解。质量损失，溶胀比和拉伸模量进行了评价。使用凝胶渗透色谱法（GPC）测量来自氢氧化钠的降解产物以验证聚合物长度和多分散性。仅在氢氧化钠和脂肪酶溶液中观察到侵蚀。然而，溶胀比和拉伸模量表明在所有含PCL的样品中体积降解。酶溶液中的可降解水凝胶在16周内显示出30%的质量损失。初始细胞毒性研究表明，对水凝胶或其降解产物无不良细胞反应。这些水凝胶具有适当的机械性能、可调的降解速率，并且由目前FDA批准的器械中的材料组成。因此，在这项研究中开发的可降解pHEMA具有相当大的潜力，作为心脏和其他应用中的组织工程支架。
Biodegradable poly(2-hydroxyethyl methacrylate) hydrogels for engineered tissue constructs were developed using atom transfer radical polymerization (ATRP), a degradable crosslinker and a macroinitiator. Hydrogels are appropriate materials for tissue engineering scaffolds due to their tissue-like mechanical compliance and mass transfer properties. However, many hydrogels that have seen wide application in medicine are not biodegradable or cannot be easily cleared from the body. Poly(2-hydroxyethyl methacrylate) (pHEMA) was selected for the scaffold material due to its reasonable mechanical strength, elasticity, long history of successful use in medicine and because it can be easily fabricated into numerous configurations. pHEMA was studied at various molecular weights between 2 kDa and 50 kDa. The molecular weight range suitable for renal clearance was an important factor in the experimental design. The fabricated hydrogels contain oligomeric blocks of polycaprolactone (PCL), a hydrolytically and enzymatically degradable polymer, as a crosslinking agent. In addition a degradable macroinitiator also containing oligomeric PCL was used to initiate the ATRP. The chain length, crosslink density, and polymerization solvent were found to greatly affect the mechanical properties of the pHEMA hydrogels. Degradation of the pHEMA hydrogels was characterized using 0.007 M NaOH, lipase solutions and phosphate buffered saline. Mass loss, swelling ratio and tensile modulus were evaluated. Degradation products from the sodium hydroxide were measured using gel permeation chromatography (GPC) to verify the polymer lengths and polydispersity. Erosion was only observed in the sodium hydroxide and lipase solutions. However, swelling ratio and tensile modulus indicate bulk degradation in all PCL containing samples. Degradable hydrogels in enzymatic solutions showed 30% mass loss in 16 weeks. Initial cell toxicity studies indicate no adverse cellular response to the hydrogels or their degradation products. These hydrogels have appropriate mechanical properties, a tunable degradation rate, and are composed of materials currently in FDA approved devices. Thus the degradable pHEMA developed in this study has considerable potential as a scaffold for tissue engineering in cardiac and other applications.
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DOI：
10.1002/jbm.820190408
发表时间：
1985-01-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
WOODWARD, SC;BREWER, PS;PITT, CG
通讯作者：
PITT, CG
影响因子：
4.9
作者：
Rice, MA;Anseth, KS
通讯作者：
Anseth, KS
影响因子：
2.8
作者：
Bakshi, A;Fisher, O;Lowman, A
通讯作者：
Lowman, A
影响因子：
5.5
作者：
SAWHNEY, AS;PATHAK, CP;HUBBELL, JA
通讯作者：
HUBBELL, JA
DOI：
10.1002/jbm.820040306
发表时间：
1970-01-01
期刊：
Journal of Biomedical Materials Research
影响因子：
--
作者：
HOMSY C A
通讯作者：
HOMSY C A