The effects of circumcision on the penis microbiome.
The effects of circumcision on the penis microbiome.
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DOI：
10.1371/journal.pone.0008422
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发表时间：
2010-01-06
期刊：
影响因子：
3.7
通讯作者：
Gray RH
中科院分区：
文献类型：
--
作者：
Price LB;Liu CM;Johnson KE;Aziz M;Lau MK;Bowers J;Ravel J;Keim PS;Serwadda D;Wawer MJ;Gray RH
包皮环切术与男性艾滋病毒、HSV-2和HPV感染的显著减少以及女性伴侣细菌性阴道病的显著减少有关。我们评估了包皮环切术前后12名HIV阴性乌干达男性的阴茎（冠状沟）微生物群。使用靶向V3-V4高变区的序列标记的16S rRNA基因焦磷酸测序来表征微生物群。使用RDP朴素贝叶斯分类器进行分类学分类。在确定的42个独特的细菌家族，假单胞菌科和草酸杆菌科是最丰富的包皮环切状态无关。包皮环切术与总体微生物群的显著变化（PerMANOVA p = 0.007）和推定的厌氧菌家族的显著减少（Wilcoxon Signed-Rank检验p = 0.014）相关。 具体而言，两个家庭-梭菌家族XI（p = 0.006）和Prevotellaceae（p = 0.006）-是唯一丰富的包皮环切术前。 在这些科中，我们鉴定了许多以前与细菌性阴道病相关的厌氧属，包括：厌氧球菌属，Finegoldia spp.，嗜胃菌属，和普雷沃氏菌属（Prevotella spp.） 包皮下间隙的缺氧微环境可能支持促炎性厌氧菌，这些厌氧菌可以激活朗格汉斯细胞，将艾滋病毒呈递给引流淋巴结中的CD 4细胞。因此，包皮环切术后厌氧菌的减少可能在预防艾滋病毒和其他性传播疾病方面发挥作用。
Circumcision is associated with significant reductions in HIV, HSV-2 and HPV infections among men and significant reductions in bacterial vaginosis among their female partners. We assessed the penile (coronal sulci) microbiota in 12 HIV-negative Ugandan men before and after circumcision. Microbiota were characterized using sequence-tagged 16S rRNA gene pyrosequencing targeting the V3–V4 hypervariable regions. Taxonomic classification was performed using the RDP Naïve Bayesian Classifier. Among the 42 unique bacterial families identified, Pseudomonadaceae and Oxalobactericeae were the most abundant irrespective of circumcision status. Circumcision was associated with a significant change in the overall microbiota (PerMANOVA p = 0.007) and with a significant decrease in putative anaerobic bacterial families (Wilcoxon Signed-Rank test p = 0.014). Specifically, two families—Clostridiales Family XI (p = 0.006) and Prevotellaceae (p = 0.006)—were uniquely abundant before circumcision. Within these families we identified a number of anaerobic genera previously associated with bacterial vaginosis including: Anaerococcus spp., Finegoldia spp., Peptoniphilus spp., and Prevotella spp. The anoxic microenvironment of the subpreputial space may support pro-inflammatory anaerobes that can activate Langerhans cells to present HIV to CD4 cells in draining lymph nodes. Thus, the reduction in putative anaerobic bacteria after circumcision may play a role in protection from HIV and other sexually transmitted diseases.
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影响因子：
15.9
作者：
de Jong, Marein A. W. P.;de Witte, Lot;Geijtenbeek, Teunis B. H.
通讯作者：
Geijtenbeek, Teunis B. H.
影响因子：
0.3
作者：
Gowe, AK;Brewer, JS
通讯作者：
Brewer, JS
影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM
DOI：
10.1007/bf00038687
发表时间：
1987-04-30
期刊：
VEGETATIO
影响因子：
--
作者：
FAITH, DP;MINCHIN, PR;BELBIN, L
通讯作者：
BELBIN, L
影响因子：
2.3
作者：
Aroutcheva, Alla;Ling, Zaodung;Faro, Sebastian
通讯作者：
Faro, Sebastian