[Effect of TSA and VPA treatment on long-tailed macaque (Macaca fascicularis)-pig interspecies somatic cell nuclear transfer].
[Effect of TSA and VPA treatment on long-tailed macaque (Macaca fascicularis)-pig interspecies somatic cell nuclear transfer].
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TSA和VPA处理对长尾猕猴(Macaca fasciculis)-猪种间体细胞核移植的影响
DOI：
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
K. Lu
中科院分区：
文献类型：
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作者：
Zu;Gao;Jun Luo;Shu;Sheng;K. Lu
长尾猕猴-猪种间体细胞核移植（iSCNT）有利于从与人类遗传背景相似的iSCNT胚胎中获得胚胎干细胞，可作为医学和基础研究的材料。本研究的主要目的是研究两种组蛋白去乙酰化酶催化剂曲古抑菌素A（TSA）和丙戊酸（VPA）的浓度和处理时间以及两种不同的胚胎培养液（PZM-3和HECM-10）对iSCNT胚胎体外发育的影响。结果表明，以PZM-3为胚胎培养液时，10 nmol/L TSA处理48 h的囊胚率显著高于对照组（22.78% vs 9.86%，P< 0.05）。然而，无论是在PZM-3还是在HECM-10中，2-10 mmol/L VPA处理都没有增加iSCNT胚胎的体外发育潜力。结果表明，TSA处理能提高长尾猕猴iSCNT胚胎的体外发育潜能。
Long-tailed macaque-pig interspecies somatic cell nuclear transfer (iSCNT) is beneficial to yield embryonic stem cells from iSCNT embryos with similar genetic background as human, which can be used as materials for medical and basic research. The primary objective of this study was to investigate the effects of concentrations and treatment duration of two histone deacetylase inhibitors-Trichostatin A (TSA) and Valproic acid (VPA) and two different embryo culture media (PZM-3 and HECM-10) on the in vitro development of iSCNT embryos. The results suggested that when PZM-3 was used as the embryo culture medium, the blastocyst rate of 10 nmol/L TSA treatment for 48 h was significantly higher than the control group (22.78% vs 9.86%, P< 0.05). However, neither in PZM-3 nor in HECM-10, 2-10 mmol/L VPA treatment did not increase the in vitro developmental potential of iSCNT embryos. It was concluded that TSA treatment could enhance the in vitro developmental potential of long-tailed macaque-pig iSCNT embryos.
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影响因子：
16
作者：
Vogelauer, M;Rubbi, L;Grunstein, M
通讯作者：
Grunstein, M
影响因子：
1.6
作者：
Zhao J;Hao Y;Ross JW;Spate LD;Walters EM;Samuel MS;Rieke A;Murphy CN;Prather RS
通讯作者：
Prather RS
影响因子：
6.1
作者：
Zheng, P;Wang, H;Ji, W
通讯作者：
Ji, W
DOI：
10.1089/153623003772032754
发表时间：
2003-12-01
期刊：
CLONING AND STEM CELLS
影响因子：
--
作者：
Lai, LX;Prather, RS
通讯作者：
Prather, RS