Hydrogen Sulfide Is Increased in Oral Squamous Cell Carcinoma Compared to Adjacent Benign Oral Mucosae.
Hydrogen Sulfide Is Increased in Oral Squamous Cell Carcinoma Compared to Adjacent Benign Oral Mucosae.
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DOI：
10.21873/anticanres.12668
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发表时间：
2018-07
影响因子：
2
通讯作者：
Shackelford RE
中科院分区：
文献类型：
--
作者：
Meram AT;Chen J;Patel S;Kim DD;Shirley B;Covello P;Coppola D;Wei EX;Ghali G;Kevil CG;Shackelford RE
硫化氢(H_2S)及其合成酶，如胱硫醚-b-合成酶、胱硫醚γ-裂解酶和3-硫代丙酮酸，在不同的人类恶性肿瘤中都会增加。由于硫化氢的半衰期很短，还没有在人类恶性肿瘤中直接测量过其浓度。在这里，我们直接测量了口腔鳞状细胞癌(OSCC)中的活体硫化氢水平。对15例口腔鳞状细胞癌和良性粘膜的穿孔活检进行了高效液相、蛋白质印迹和组织芯片分析。口腔鳞癌组织中H_2S浓度明显高于癌旁良性口腔粘膜组织。免疫印迹和组织芯片研究显示，口腔鳞癌组织中胱硫醚b合成酶、胱硫醚γ裂解酶、3-硫代丙酮酸、磷酸化STAT3、丝裂原活化蛋白、端粒酶逆转录酶和丝裂原活化蛋白的蛋白水平显著升高。口腔鳞状细胞癌中硫化氢浓度和合成硫化氢的酶显著增加。在这里，第一次测量了活体人类恶性肿瘤内的硫化氢浓度，并与邻近的良性组织进行了比较。
Hydrogen sulfide (H2S) and the enzymes that synthesize it, cystathionine-b-synthase, cystathionine γ-lyase, and 3-mercaptopyruvate, are increased in different human malignancies. Due to its short half-life, H2S concentrations have not been directly measured in a human malignancy. Here we directly measured in vivo H2S levels within oral squamous cell carcinoma (OSCC). Punch biopsies of OSCC and benign mucosae from 15 patients were analyzed by HPLC, western blotting, and tissue microarray analyses. H2S concentrations were significantly higher in OSCC compared to adjacent benign oral mucosae. Western blot and tissue microarray studies revealed significantly increased cystathionine-b-synthase, cystathionine γ-lyase, and 3-mercaptopyruvate, phopho-Stat3, mitoNEET, hTERT, and MAPK protein levels in OSCC. H2S concentrations and the enzymes that synthesize it are significantly increased in OSCC. Here, for the first time H2S concentrations within a living human malignancy were measured and compared to adjacent counterpart benign tissue.
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影响因子：
20.1
作者：
Calvert JW;Jha S;Gundewar S;Elrod JW;Ramachandran A;Pattillo CB;Kevil CG;Lefer DJ
通讯作者：
Lefer DJ
影响因子：
--
作者：
Shackelford, Rodney E;Mayhall, Kim;Coppola, Domenico
通讯作者：
Coppola, Domenico
DOI：
10.1016/j.ijom.2014.10.001
发表时间：
2015-05-01
影响因子：
2.4
作者：
Fu, X.;Feng, Y.
通讯作者：
Feng, Y.
影响因子：
3.5
作者：
Jurkowska, Halina;Placha, Wojciech;Wrobel, Maria
通讯作者：
Wrobel, Maria
影响因子：
2.9
作者：
Jackson, Michael R.;Melideo, Scott L.;Jorns, Marilyn Schuman
通讯作者：
Jorns, Marilyn Schuman