An Affinity Capillary Electrophoresis for the Separation of Sequence Isomers of Oligonucleotide

An Affinity Capillary Electrophoresis for the Separation of Sequence Isomers of Oligonucleotide
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亲和毛细管电泳分离寡核苷酸序列异构体

DOI:
10.1246/cl.2000.106
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发表时间:
2000
期刊:
影响因子:
1.6
通讯作者:
M. Maeda
M. Maeda
中科院分区:
化学4区
文献类型:
--
作者:
Y. Katayama;Takumi Arisawa;Y. Ozaki;M. Maeda

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建立了一种以磷脂酰肌醇-聚丙烯酰胺偶联物为假固定化亲和配基的基因突变检测方法。在该体系中,镁离子浓度被发现是一个关键因素,以实现有效的分离序列异构体的寡核苷酸具有相同的链长。
An affinity capillary electrophoresis for gene mutation assay was developed using oligonucleotide-polyacrylamide conjugate as pseudo-immobilized affinity ligand. In the system, magnesium ion concentration was found to be a key factor to achieve efficient separation of sequential isomers of oligonucleotide with the same chain length.
通过毛细管亲和凝胶电泳对寡脱氧核苷酸进行碱基特异性分离
DOI: --
发表时间: 1998
期刊: Electrophoresis
影响因子: 2.9
作者:
Y. Baba;T. Sawa;A. Kishida;M. Akashi
通讯作者: M. Akashi