The aerolysin nanopore: from peptidomic to genomic applications.

The aerolysin nanopore: from peptidomic to genomic applications.
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DOI:
10.1039/c8nr04255a
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发表时间:
2018-08-07
期刊:
影响因子:
6.7
通讯作者:
Tian K
Tian K
中科院分区:
材料科学2区
文献类型:
--
作者:
Wang Y;Gu LQ;Tian K

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气溶素孔(ARP)是一种新兴的纳米孔,已广泛用于多肽和蛋白质传感。近年来,一些研究小组探索了ARP在遗传和表观遗传标记检测中的应用。本文简要综述了ARP的应用现状,从肽组检测到基因组检测;最近报道的ARP的定点诱变;和新的基因组DNA传感方法,以及它们的优缺点。本文还对ARP在核酸测序和生物分子传感方面的应用前景进行了展望。
The aerolysin pore (ARP) is a newly emerging nanopore that has been extensively used for peptide and protein sensing. Recently, several groups have explored the application of ARP in detecting genetic and epigenetic markers. This brief review summarizes the current applications of ARP, progressing from peptidomic to genomic detection; the recently reported site-directed mutagenesis of ARP; and new genomic DNA sensing approaches, and their advantages and disadvantages. This review will also discuss the perspectives and future applications of ARP for nucleic acid sequencing and biomolecule sensing.
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