Inhibition of HSV-1 by chemoattracted neutrophils: supernatants of corneal epithelial cells (HCE) and macrophages (THP-1) treated with virus components chemoattract neutrophils (PMN), and supernatants of PMN treated with these conditioned media inhibit viral growth.
Inhibition of HSV-1 by chemoattracted neutrophils: supernatants of corneal epithelial cells (HCE) and macrophages (THP-1) treated with virus components chemoattract neutrophils (PMN), and supernatants of PMN treated with these conditioned media inhibit viral growth.
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通过趋化性嗜中性粒细胞抑制HSV-1：角膜上皮细胞（HCE）和巨噬细胞（THP-1）用病毒成分进行了趋化性嗜中性粒细胞（PMN）的巨噬细胞（THP-1），以及用这些条件培养基抑制病毒抑制病毒抑制病毒生长的PMN的级别的级别的上网。
DOI：
10.1007/s00705-012-1306-y
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发表时间：
2012-07
影响因子：
2.7
通讯作者：
Hooks, John J.
中科院分区：
文献类型：
--
作者：
Hayashi, Kozaburo;Hooper, Laura C.;Okuno, Toshiomi;Takada, Yuichiro;Hooks, John J.
用体外系统研究了中性粒细胞在角膜疱疹中的作用。人角膜细胞(HCE)和巨噬细胞(THP-1)感染HSV-1或经病毒成分(DNA或病毒免疫复合体)处理后释放趋化因子，从而吸引PMN。在中性粒细胞中检测到高活性氧物种。当PMN覆盖在感染的HCE细胞上(50：1)时，可抑制HSV。中性粒细胞与经病毒成分处理的HCE细胞上清液孵育后，释放出过氧化氢和髓过氧化物酶。这些都抑制了病毒的生长。PMN释放NO和MIG，可能使CD4T细胞分化为Th1。中性粒细胞参与先天免疫反应，限制病毒生长，并启动免疫病理学。
The role of PMNs (neutrophils) in corneal herpes was studied using an in vitro system. Human corneal cells (HCE) and macrophages (THP-1) infected with HSV-1 or treated with virus components (DNA or virus immune complexes) released chemokines, which attracted PMNs. Highly reactive oxygen species were detected in PMNs. PMNs inhibited HSV when overlaid onto infected HCE cells (50:1). PMNs incubated with the supernatants of HCE cells treated with virus components released H2O2 and myeloperoxidase. These inhibited virus growth. PMNs released NO and MIG, which may differentiate CD4 T cells to Th1. PMNs participate in innate immune responses, limit virus growth, and initiate immunopathology.
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