Protective effects of quercetin, polydatin, and folic acid and their mixtures in a zebrafish (Danio rerio) fetal alcohol spectrum disorder model.
Protective effects of quercetin, polydatin, and folic acid and their mixtures in a zebrafish (Danio rerio) fetal alcohol spectrum disorder model.
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DOI：
10.1016/j.ntt.2020.106928
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发表时间：
2020-11
影响因子：
2.9
通讯作者：
Marrs JA
中科院分区：
文献类型：
--
作者：
Cadena PG;Sales Cadena MR;Sarmah S;Marrs JA
以斑马鱼为动物模型，研究了槲皮素、虎杖苷和叶酸及其混合物对胎儿酒精谱系障碍的保护作用。将斑马鱼胚胎暴露于150 mM乙醇6或22 h，并用QUE、POL、FA及其混合物（37.5 - 100.0 μM）共处理。评价了上皮进展、致畸作用和行为。乙醇暴露减少epiboly，和FA和QUE保护这些乙醇引起的缺陷。POL没有减少外包缺陷。QUE+FA混合物显示出可能的拮抗作用。在所有乙醇暴露组中观察到的致畸作用相似。QUE、FA和QUE+POL降低了受影响动物的百分比，但处理并未消除致畸作用。行为测量分为小（4和8毫米/秒之间）和高游泳活动（> 8毫米/秒）。当暴露于强光时，与对照组和乙醇组相比，所有实验组的小型游泳活动都有所减少。此外，暴露于乙醇的幼虫比对照组受到更多的抑制，在高游泳活动中没有显示出适应期（实验60分钟后）。化学治疗，如QUE和POL减少乙醇暴露引起的行为缺陷。总之，本研究提出了新的证据，QUE，POL，FA和它们的混合物部分保护epiboly，致畸，和行为缺陷引起的乙醇暴露。QUE、FA和QUE+POL在减少这些缺陷方面比其他研究的化合物和混合物更有效。
Protective effects of quercetin (QUE), polydatin (POL), and folic acid (FA) and their mixtures were tested using zebrafish to model fetal alcohol spectrum disorder in this study. Zebrafish embryos were exposed to 150 mM ethanol for 6 or 22 h and co-treated with QUE, POL, FA, and their mixtures (37.5 – 100.0 μM). Epiboly progression, teratogenic effects, and behavior were evaluated. Ethanol exposure reduced epiboly, and FA and QUE protected against these ethanol-induced defects. POL did not reduce epiboly defects. The mixture QUE+FA showed a possible antagonistic effect. The observed teratogenic effects were similar in all ethanol exposed groups. QUE, FA and QUE+POL reduced the percentage of affected animals, but treatments did not eliminate teratogenic effects. Behavioral measurements were divided into small (between 4 and 8 mm/s) and high swimming activity (> 8 mm/s). All experimental groups displayed a reduction in small swimming activity as compared to control and ethanol groups when exposed to bright light. Additionally, larvae exposed to ethanol were more inhibited than control, not showing a habituation period (after 60 min of experiment) in high swimming activity. Chemical treatments like QUE and POL reduced behavioral defects induced by ethanol exposure. In conclusion, this study presents new evidence that QUE, POL, FA and their mixtures partially protected epiboly, teratogenic, and behavioral defects induced by ethanol exposure. QUE, FA and QUE+POL were more effective in reducing these defects than the other studied compounds and mixtures.
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影响因子：
--
作者：
Lee MS;Bonner JR;Bernard DJ;Sanchez EL;Sause ET;Prentice RR;Burgess SM;Brody LC
通讯作者：
Brody LC
影响因子：
3.3
作者：
Muralidharan P;Sarmah S;Zhou FC;Marrs JA
通讯作者：
Marrs JA
影响因子：
3.4
作者：
MacPhail, R. C.;Brooks, J.;Padilla, S.
通讯作者：
Padilla, S.
影响因子：
5.8
作者：
Caddeo, Carla;Nacher, Amparo;Manconi, Maria
通讯作者：
Manconi, Maria
影响因子：
120.7
作者：
May, Philip A.;Chambers, Christina D.;Hoyme, H. Eugene
通讯作者：
Hoyme, H. Eugene