The establishment of a chemically defined serum-free culture system for human dental pulp stem cells.
The establishment of a chemically defined serum-free culture system for human dental pulp stem cells.
复制标题
人牙髓干细胞化学成分确定的无血清培养体系的建立
DOI：
10.1186/s13287-018-0928-8
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发表时间：
2018-07-11
影响因子：
7.5
通讯作者：
Guo W
中科院分区：
文献类型：
--
作者：
Xiao J;Yang D;Li Q;Tian W;Guo W
BackgroundThe concept of establishing a dental stem cell (DSC) bank for oral and maxillofacial regeneration has become of great interest but it remains at a primitive stage. The routine application of serum-containing conditions for human DSC (hDSC) culture is in great controversy considering that the animal-originated serum can cause serious ethical concerns and lead to increasingly irrelevant variables, errors, and poor repeatability of experiment results. Thus, this study aimed to establish a safe, stable and efficient hDSC serum-free culturing system for future DSC bank usage.MethodsDental pulp stem cells (DPSCs) from human permanent tooth pulp were isolated, expanded, passaged, and divided into two groups according to their culture conditions: group 1 was the serum-containing medium (SCM) group; and group 2 was the serum-free Essential 8 medium (E8) group. DPSCs were characterized first, followed by cell proliferation, pluripotency, and migration study in SCM and E8 medium.ResultsHuman DPSCs (hDPSCs) in E8 medium demonstrated greater proliferation, pluripotency, migration ability and less apoptosis. hDPSCs could be successfully induced to the adipogenic, osteogenic, neurogenic, and chondrogenic lineages in E8 group. Real-time polymerase chain reaction indicated that the expression of PPAR-γ, RUNX2, OCN and MAP-2 was higher in E8 group.ConclusionsCompared with serum-containing medium, E8 medium exhitibed higher ability in maintaining the cell proliferation, pluripotency, migration, and stability. This new serum-free culture environment might be applicable for hDSC culture in the future.
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影响因子：
0.9
作者：
Calne, RY
通讯作者：
Calne, RY
影响因子：
4.5
作者：
Al-Saqi, Shahla Hamza;Saliem, Mohammed;Gotherstrom, Cecilia
通讯作者：
Gotherstrom, Cecilia
影响因子：
64.8
作者：
Dever DP;Bak RO;Reinisch A;Camarena J;Washington G;Nicolas CE;Pavel-Dinu M;Saxena N;Wilkens AB;Mantri S;Uchida N;Hendel A;Narla A;Majeti R;Weinberg KI;Porteus MH
通讯作者：
Porteus MH
影响因子：
20.3
作者：
GRONTHOS, S;SIMMONS, PJ
通讯作者：
SIMMONS, PJ
DOI：
10.1016/j.bbrc.2007.02.057
发表时间：
2007-04-20
影响因子：
3.1
作者：
Chen, Tain-Hsiung;Chen, Wei-Ming;Hung, Shih-Chieh
通讯作者：
Hung, Shih-Chieh