Involvement of estradiol-17beta and its membrane receptor, G protein coupled receptor 30 (GPR30) in regulation of oocyte maturation in zebrafish, Danio rario.
Involvement of estradiol-17beta and its membrane receptor, G protein coupled receptor 30 (GPR30) in regulation of oocyte maturation in zebrafish, Danio rario.
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DOI：
10.1016/j.ygcen.2008.10.003
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发表时间：
2009-03
影响因子：
2.7
通讯作者：
Thomas, Peter
中科院分区：
文献类型：
--
作者：
Pang, Yefei;Thomas, Peter
孤儿G蛋白偶联受体GPR30是大西洋大黄鱼卵母细胞上的一种高亲和力、特异的雌激素膜受体，介导雌激素抑制这种波斯形鱼类卵母细胞的成熟。为了确定这些发现对其他硬骨鱼的广泛适用性，在本研究中，对鲤科鱼类斑马鱼进行了类似的实验。GPR30在斑马鱼卵母细胞中有表达，但在卵泡细胞中未见表达。17，20β-二氢氧-4-孕烯-3-酮(DHP)与17，20-二氢-4-孕烯-3-酮(β)或GPR30激动剂ICI182780、他莫昔芬或GPR30特异性激动剂G-1共同孵育的斑马鱼卵母细胞自发成熟和17，20-二氢-4-孕烯-3-酮(DHP)诱导的成熟均显著降低。另一方面，当斑马鱼卵泡与芳香酶抑制剂ATD孵育时，自发卵母细胞成熟率增加了两倍。此外，ATD对生发泡破裂(GVBD)的刺激作用可被100 nM的E_2或G_1部分逆转。这些结果表明，通过GPR30作用的内源雌激素参与了斑马鱼卵母细胞减数分裂停滞的维持。
The orphan G protein coupled receptor, GPR30, has the characteristics of a high affinity, specific estrogen membrane receptor on Atlantic croaker oocytes and mediates estrogen inhibition of oocyte maturation in this perciform fish. In order to determine the broad applicability of these findings to other teleosts, similar experiments were conducted in a cyprinid fish, zebrafish, in the present study. GPR30 mRNA expression was detected in zebrafish oocytes but not in the ovarian follicular cells. Both spontaneous and 17, 20β-dihyroxy-4-pregnen-3-one (DHP)-induced maturation of follicle-enclosed zebrafish oocytes was significantly decreased when they were incubated with either estradiol-17β, or the GPR30 agonists, ICI 182 780 and tamoxifen, or with the GPR30 specific agonist G-1. On the other hand spontaneous oocyte maturation increased two-fold when zebrafish ovarian follicles were incubated with an aromatase inhibitor, ATD. Moreover, the stimulatory effects of ATD on germinal vesicle breakdown (GVBD) were partially reversed by co-treatment with 100 nM of E2 or G-1. These results suggest that endogenous estrogens acting through GPR30 are involved in maintaining meiotic arrest of zebrafish oocytes.
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影响因子：
2.7
作者：
SAKAI, N;IWAMATSU, T;NAGAHAMA, Y
通讯作者：
NAGAHAMA, Y
影响因子：
--
作者：
Filardo, EJ;Quinn, JA;Bland, KI
通讯作者：
Bland, KI
影响因子：
2.7
作者：
Thomas, P;Zhu, Y;Pace, M
通讯作者：
Pace, M
影响因子：
4.8
作者：
Thomas, P;Pang, Y;Dong, J
通讯作者：
Dong, J
影响因子：
4.8
作者：
Wang, Cheng;Prossnitz, Eric R.;Roy, Shyamal K.
通讯作者：
Roy, Shyamal K.