Studies of translational misreading in vivo show that the ribosome very efficiently discriminates against most potential errors.
Studies of translational misreading in vivo show that the ribosome very efficiently discriminates against most potential errors.
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DOI：
10.1261/rna.039792.113
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发表时间：
2014-01
期刊：
影响因子：
--
通讯作者：
Farabaugh PJ
中科院分区：
文献类型：
--
作者：
Manickam N;Nag N;Abbasi A;Patel K;Farabaugh PJ
众所周知，蛋白质合成以高保真度发生。本文描述了一个灵敏的系统，用于分析多个 tRNA 产生的所有可能错义错误的频率。当密码子-反密码子配对涉及具有与规范沃森-克里克碱基对相似的几何形状的非规范碱基配对时，观察到唯一以明显频率发生的错误。蛋白质合成必须快速、重复地区分单个正确的氨酰基-tRNA 和许多不正确的氨酰基-tRNA。我们尝试通过 tRNA、tRNA 和 tRNA 测量所有可能的错义错误的频率。最常见的错误涉及三种类型的错配核苷酸对：U•U、U•C 或U•G，所有这些都可以形成几何形状与规范U•A 或C•G 沃森-克里克对类似的非规范碱基对。我们的系统足够灵敏，可以测量其他潜在错配的错误，这些错配的发生频率低至 500,000 个密码子中的 1 个。核糖体似乎能够有效地区分任何具有非沃森-克里克几何形状的核糖对。为了区分涉及近沃森-克里克配对的错误，这种极端的准确性可能是必要的。
It is well established that protein synthesis occurs with high fidelity. This paper describes a sensitive system to analyze the frequency of all possible missense errors by several tRNAs. The only errors that occur with appreciable frequency are observed when codon–anticodon pairing involves noncanonical base-pairing with geometries similar to canonical Watson–Crick base pairs. Protein synthesis must rapidly and repeatedly discriminate between a single correct and many incorrect aminoacyl-tRNAs. We have attempted to measure the frequencies of all possible missense errors by tRNA, tRNA and tRNA. The most frequent errors involve three types of mismatched nucleotide pairs, U•U, U•C, or U•G, all of which can form a noncanonical base pair with geometry similar to that of the canonical U•A or C•G Watson–Crick pairs. Our system is sensitive enough to measure errors at other potential mismatches that occur at frequencies as low as 1 in 500,000 codons. The ribosome appears to discriminate this efficiently against any pair with non-Watson–Crick geometry. This extreme accuracy may be necessary to allow discrimination against the errors involving near Watson–Crick pairing.
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影响因子：
5.6
作者：
SPRINGGATE, CF;LOEB, LA
通讯作者：
LOEB, LA
影响因子：
14.9
作者：
Leontis, NB;Stombaugh, J;Westhof, E
通讯作者：
Westhof, E
DOI：
10.1007/bf00332630
发表时间：
1982-01-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
ANDERSSON, DI;BOHMAN, K;KURLAND, CG
通讯作者：
KURLAND, CG
影响因子：
2.9
作者：
Juers, DH;Hakda, S;Huber, RE
通讯作者：
Huber, RE
影响因子：
4.5
作者：
Kramer, Emily B.;Farabaugh, Philip J.
通讯作者：
Farabaugh, Philip J.