Tissue-specific expression of the human prostate-specific antigen gene in transgenic mice: implications for tolerance and immunotherapy.
Tissue-specific expression of the human prostate-specific antigen gene in transgenic mice: implications for tolerance and immunotherapy.
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转基因小鼠中人类前列腺特异性抗原基因的组织特异性表达：对耐受性和免疫治疗的影响。
DOI：
10.1073/pnas.94.12.6369
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发表时间：
1997
影响因子：
11.1
通讯作者：
Frelinger,JG
中科院分区：
文献类型：
--
作者：
Wei,C;Willis,RA;Tilton,BR;Looney,RJ;Lord,EM;Barth,RK;Frelinger,JG
Human prostate-specific antigen (PSA) has been widely used as a serum marker for cancer of the prostate. The cell type-specific expression of PSA also makes it a potential tumor antigen for prostate cancer immunotherapy. Study of the immunological aspects of PSA within either normal or malignant prostate tissue has been hampered by the lack of a mouse model, because no PSA counterpart has been identified in mice. Using a 14-kb genomic DNA region that encompasses the entire human PSA gene and adjacent flanking sequences, we generated a series of human PSA transgenic mice. In the six independent lines of transgenic mice generated, the expression of the human PSA transgene, driven by its owncis-acting regulatory elements, is specifically targeted to the prostate. Tissue distribution analysis demonstrated that PSA transgene expression closely follows the human expression pattern. Immunohistochemical analysis of the prostate tissue also showed that the expression of the PSA transgene is confined to the ductal epithelial cells. Despite expressing PSA as a self-antigen in the prostate, these transgenic mice were able to mount a cytotoxic immune response against PSA expressed by tumor cells, indicating that expression of the transgene has not resulted in complete nonresponsiveness. This transgenic mouse model will provide a well defined system to gain an insight into the mechanisms of nonresponsiveness to PSA, ultimately leading to strategies for immunotherapy of human prostate cancer using PSA as the target antigen.
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DOI：
10.1084/jem.179.3.1005
发表时间：
1994-03-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Bakker AB;Schreurs MW;de Boer AJ;Kawakami Y;Rosenberg SA;Adema GJ;Figdor CG
通讯作者：
Figdor CG
免疫能力与恶性小鼠肺肿瘤的皮下生长速度之间的直接关系。
影响因子：
11.2
作者：
J. Yuhas;N. Pazmiño;J. Proctor;R. Toya
通讯作者：
R. Toya
影响因子：
4.3
作者：
H. Sambrook
通讯作者：
H. Sambrook
编码由 HLA-A31 限制性肿瘤浸润淋巴细胞识别的黑色素瘤肿瘤抗原的基因的鉴定 [已发表的勘误表出现在 J Exp Med 1995 Mar 1;181(3):1261]
影响因子：
15.3
作者：
Rongfu Wang;P. Robbins;Yutaka Kawakami;X. Kang;Steven A. Rosenberg
通讯作者：
Steven A. Rosenberg
DOI：
10.1073/pnas.92.8.3439
发表时间：
1995-04-11
影响因子：
11.1
作者：
GREENBERG, NM;DEMAYO, F;ROSEN, JM
通讯作者：
ROSEN, JM