Fractionation of feline bone marrow with the soybean agglutinin lectin yields populations enriched for erythroid and myeloid elements: transplantation of soybean agglutinin-negative cells into lethally irradiated recipients.
Fractionation of feline bone marrow with the soybean agglutinin lectin yields populations enriched for erythroid and myeloid elements: transplantation of soybean agglutinin-negative cells into lethally irradiated recipients.
复制标题
用大豆凝集素凝集素对猫骨髓进行分级，产生富含红细胞和骨髓元素的群体：将大豆凝集素阴性细胞移植到致命辐射的受体中。
DOI：
10.1097/00007890-199708150-00022
复制
发表时间：
1997
期刊：
影响因子：
6.2
通讯作者：
Yamamoto,JK
中科院分区：
文献类型：
--
作者：
Gengozian,N;Reyes,L;Pu,R;Homer,BL;Bova,FJ;Yamamoto,JK
用大豆凝集素 (SBA) 凝集素处理的猫骨髓细胞被分为两个群体，凝集的 SBA (+) 部分主要包含骨髓来源的细胞，非凝集的 SBA (-) 部分主要由红系细胞组成。 FACScan 分析揭示了基于光散射特性的细胞的明显区别，即在 SBA (+) 级分中发现了大细胞和高粒度细胞，而在 SBA (-) 级分中发现了具有低前向光散射和侧向光散射的细胞。集落形成试验表明，集落形成单位粒细胞/单核细胞 (CFU-GM) 细胞对 SBA 具有很强的亲和力，因为这些细胞几乎全部存在于 SBA (+) 部分中；相反，突发形成红系单位（BFU-E）形成细胞集中在SBA（-）级分中。当通过逆流离心淘洗 (CCE) 分离骨髓时，发现 CFU-GM 形成细胞与 SBA 的结合存在差异。以 21 和 25 ml/min 收集的细胞的 SBA (-) 级分主要含有 BFU-E 形成细胞，类似于用全骨髓观察到的情况；随后的 CCE 级分（以 32 ml/min 收集的级分和转子关闭级分）在用 SBA 处理时显示 SBA (-) 级分中存在少量但显着数量的 CFU-GM 细胞。 T 淋巴细胞主要存在于全骨髓的 SBA (+) 部分和 CCE 部分中。使用早期 CCE SBA (-) 部分成功进行了自体骨髓移植。后一种细胞用于我们最初的移植尝试，因为我们实验室正在进行的研究表明，当从感染猫免疫缺陷病毒的动物中获得骨髓时，这些细胞不含任何含有病毒的细胞。总之，虽然猫骨髓的 SBA 处理产生了 CFU-GM 和 BFU-E 祖细胞的显着分离，但选择的 CCE SBA (-) 级分含有能够为受到致命辐射的动物提供造血重建的干细胞。
Feline bone marrow cells treated with the soybean agglutinin (SBA) lectin are separated into two populations, the agglutinated SBA (+) fraction containing predominantly cells of myeloid origin and the nonagglutinated SBA (-) fraction consisting of cells primarily of the erythroid lineage. FACScan analyses revealed a clear distinction of the cells based on their light scattering properties, ie, large cells and cells with high granularity were found in the SBA (+) fraction, whereas cells having a low forward light scatter and side light scatter were found in the SBA (-) fraction. Colony-forming assays showed colony-forming unit-granulocyte/monocyte (CFU-GM) cells to have a strong affinity for SBA because these were found almost entirely in the SBA (+) fraction; in contrast, burst-forming unit-erythroid (BFU-E)-forming cells were concentrated in the SBA (-) fraction. When the marrow was fractionated by counterflow centrifugal elutriation (CCE), a differential binding to SBA among the CFU-GM forming cells was found. The SBA (-) fractions of cells collected at 21 and 25 ml/min contained primarily BFU-E forming cells, similar to that observed with whole marrow; the later CCE fractions, those collected at 32 ml/min and the rotor off fraction, when treated with SBA showed a small but significant number of CFU-GM cells in the SBA (-) fraction. T lymphocytes were found predominantly in the SBA (+) fractions of whole bone marrow and the CCE fractions. Successful autologous marrow transplants were performed with the early CCE SBA (-) fractions. The latter cells were used for our initial transplant attempts because ongoing studies in our laboratory had shown these cells to be free of any viral-containing cells when the marrow had been obtained from animals infected with the feline immunodeficiency virus. In summary, although SBA treatment of feline marrow yields a marked separation of CFU-GM and BFU-E progenitors, select CCE SBA (-) fractions contain stem cells capable of providing hematopoietic reconstitution of lethally irradiated animals.
登录
查看更多内容
使用大豆凝集素从鼠和人骨髓中富集 CFU-C。
影响因子：
20.3
作者：
Reisner,Y;Kapoor,N;Hodes,MZ;O'Reilly,RJ;Good,RA
通讯作者：
Good,RA
通过逆流离心淘洗分离猫骨髓细胞：鉴定和分离推定的早期和晚期骨髓/红系祖细胞
影响因子：
6.2
作者：
N. Gengozian;A. Legendre
通讯作者：
A. Legendre
对注射自体骨髓的致死辐照恒河猴进行 T 辅助细胞、T 抑制细胞和 B 淋巴细胞的功能评估。
影响因子：
6.2
作者：
Gengozian,N;Moriarty,C;Good,RA
通讯作者：
Good,RA
使用大豆凝集素分离小鼠 B 和 T 淋巴细胞。
DOI：
--
发表时间：
1976
期刊：
Biochemical and Biophysical Research Communications - BBRC
影响因子：
--
作者：
Y. Reisner;A. Ravid;N. Sharon
通讯作者：
N. Sharon
豚鼠和沙鼠红细胞花结，在猫的血液、骨髓和胸腺中具有不同的细胞。
影响因子：
4.3
作者：
Gengozian,N;Good,RA;Day,NK
通讯作者：
Day,NK