Characterisation of murine MICL (CLEC12A) and evidence for an endogenous ligand.
Characterisation of murine MICL (CLEC12A) and evidence for an endogenous ligand.
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DOI：
10.1002/eji.200738057
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发表时间：
2008-04
影响因子：
5.4
通讯作者：
Brown, Gordon D.
中科院分区：
文献类型：
--
作者：
Pyz, Elwira;Huysamen, Cristal;Marshall, Andrew S. J.;Gordon, Siamon;Taylor, Philip R.;Brown, Gordon D.
抑制性受体是控制细胞激活所必需的，它们在调节体内平衡和免疫方面起着至关重要的作用。我们以前发现了一种人类抑制性C型凝集素样受体MICL(Clec12a)，它是一种高度糖基化的单体，主要表达在髓系细胞上。在这里，我们描述了MICL的小鼠同源基因(MMICL)，并证明了该受体在结构和功能上与人类同源基因(HMICL)相似，尽管有一些显著的差异。MMICL以二聚体的形式表达，糖基化程度不高；然而，与hMICL一样，该受体可以在激活时招募抑制性磷酸酶，并在选定的TLR激动剂刺激下下调白细胞的表达。利用新的单抗，我们证明，与人类受体一样，mMICL主要由髓系细胞表达。然而，外周血中的B细胞和CD8+T细胞以及骨髓中的NK细胞也表达mMICL。最后，我们发现mMICL识别多种小鼠组织中的内源性配体，表明该受体在体内平衡中发挥作用。
Inhibitory receptors are required for the control of cellular activation and they play essential roles in regulating homeostasis and immunity. We previously identified a human inhibitory C-type lectin-like receptor, MICL (CLEC12A), a heavily glycosylated monomer predominantly expressed on myeloid cells. Here we characterise the murine homolog of MICL (mMICL), and demonstrate that the receptor is structurally and functionally similar to the human orthologue (hMICL), although there are some notable differences. mMICL is expressed as a dimer and is not heavily glycosylated; however, like hMICL, the receptor can recruit inhibitory phosphatases upon activation, and is down-regulated on leukocytes following stimulation with selected TLR agonists. Using novel monoclonal antibodies, we demonstrate that, like the human receptor, mMICL is predominantly expressed by myeloid cells. However, mMICL is also expressed by B cells and CD8+ T cells in peripheral blood, and NK cells in the bone marrow. Finally, we show that mMICL recognises an endogenous ligand in a variety of murine tissues, suggesting that the receptor plays a role in homeostasis.
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