Immunogenic properties of archaeal species found in bioaerosols.
Immunogenic properties of archaeal species found in bioaerosols.
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DOI：
10.1371/journal.pone.0023326
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Marsolais D
中科院分区：
文献类型：
--
作者：
Blais Lecours P;Duchaine C;Taillefer M;Tremblay C;Veillette M;Cormier Y;Marsolais D
生物气溶胶相关的肺部疾病的病因仍然知之甚少。最近，古细菌出现了突出的农业环境的空气中的成分，但呼吸道暴露于古细菌的后果仍然未知。由于古细菌的亚组分可以是免疫原性的，我们使用小鼠模型来研究对农业设施中发现的两种古细菌物种的肺免疫应答：史氏甲烷短杆菌（MBS）和斯塔曼甲烷球菌（MSS）。小鼠鼻内给予6.25、25或100 µg MBS或MSS，每日一次，每周3天，持续3周。MSS诱导更严重的组织病理学改变比MBS与血管周围的粒细胞积累，肺泡隔的明显增厚，肺泡巨噬细胞积累和血管周围单核细胞积累增加。支气管肺泡灌洗液分析显示，MSS的白细胞蓄积是MBS的3倍。滴注100 µg MBS或MSS导致单核细胞/巨噬细胞主要蓄积（分别为4.5×105和4.8×105个细胞/ml），然后是CD 4 + T细胞（分别为1.38×105和1.94×105个细胞/ml），B细胞CD 8 + T细胞（分别为0.20 × 105和0.31×105 cells/ml）。两种古细菌在血浆中诱导相似的抗原特异性IgG滴度。MSS而不是MBS引起肺中嗜酸性粒细胞和中性粒细胞的积累，这令人惊讶地与接种物的大小成反比。与MBS相比，MSS的免疫原性更强，通过气道中髓样树突状细胞的3倍更高的积累来证实。因此，古细菌的剂量和种类决定了肺免疫应答的程度和性质。这是第一次报告的免疫调节作用的古细菌物种中发现的生物气溶胶。
The etiology of bioaerosol-related pulmonary diseases remains poorly understood. Recently, archaea emerged as prominent airborne components of agricultural environments, but the consequences of airway exposure to archaea remain unknown. Since subcomponents of archaea can be immunogenic, we used a murine model to study the pulmonary immune responses to two archaeal species found in agricultural facilities: Methanobrevibacter smithii (MBS) and Methanosphaera stadtmanae (MSS). Mice were administered intranasally with 6.25, 25 or 100 µg of MBS or MSS, once daily, 3 days a week, for 3 weeks. MSS induced more severe histopathological alterations than MBS with perivascular accumulation of granulocytes, pronounced thickening of the alveolar septa, alveolar macrophages accumulation and increased perivascular mononucleated cell accumulation. Analyses of bronchoalveolar lavage fluids revealed up to 3 times greater leukocyte accumulation with MSS compared to MBS. Instillation of 100 µg of MBS or MSS caused predominant accumulation of monocyte/macrophages (4.5×105 and 4.8×105 cells/ml respectively) followed by CD4+ T cells (1.38×105 and 1.94×105 cells/ml respectively), B cells (0.73×105 and 1.28×105 cells/ml respectively), and CD8+ T cells (0.20×105 and 0.31×105 cells/ml respectively) in the airways. Both archaeal species induced similar titers of antigen-specific IgGs in plasma. MSS but not MBS caused an accumulation of eosinophils and neutrophils in the lungs, which surprisingly, correlated inversely with the size of the inoculum. Stronger immunogenicity of MSS was confirmed by a 3 fold higher accumulation of myeloid dendritic cells in the airways, compared to MBS. Thus, the dose and species of archaea determine the magnitude and nature of the pulmonary immune response. This is the first report of an immunomodulatory role of archaeal species found in bioaerosols.
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影响因子：
15.9
作者：
Lambrecht, BN;De Veerman, M;Pauwels, RA
通讯作者：
Pauwels, RA
DOI：
10.1164/rccm.200210-1154oc
发表时间：
2004-04-15
影响因子：
24.7
作者：
Blanchet, MR;Israël-Assayag, E;Cormier, Y
通讯作者：
Cormier, Y
影响因子：
11.4
作者：
HUET, J;SCHNABEL, R;ZILLIG, W
通讯作者：
ZILLIG, W
DOI：
10.1164/ajrccm.159.4.9807085
发表时间：
1999-04-01
影响因子：
24.7
作者：
Dakhama, A;Hegele, RG;Cormier, Y
通讯作者：
Cormier, Y
影响因子：
15.9
作者：
Gudmundsson, G;Hunninghake, GW
通讯作者：
Hunninghake, GW