Preparation and electrophoretic separation of Bodipy-Fl-labeled glycosphingolipids.
Preparation and electrophoretic separation of Bodipy-Fl-labeled glycosphingolipids.
复制标题
DOI：
10.1016/j.chroma.2012.01.031
复制
发表时间：
2012-03-16
期刊：
影响因子：
--
通讯作者：
Dovichi NJ
中科院分区：
文献类型：
--
作者：
Sarver SA;Keithley RB;Essaka DC;Tanaka H;Yoshimura Y;Palcic MM;Hindsgaul O;Dovichi NJ
用荧光团Bodipy-Fl标记几种鞘糖脂，并使用毛细管电泳与激光诱导荧光检测进行分析。以Bodipy-Fl的N-羟基琥珀酰亚胺酯为原料，通过酰化反应合成了GM 1-、LacCer-和Cer-Bodipy-Fl，并利用酶法合成了其他几种鞘糖脂，包括GT 1a-、GD 1a-、GM 2-、GM 3-、GD 3-和GlcCer-Bodipy-Fl。采用TRIS/CHES/SDS/α-环糊精缓冲液的胶束电动毛细管色谱比已建立的硼酸盐/脱氧胆酸盐/甲基-β-环糊精缓冲液的分离效果更好。九种Bodipy-Fl标记的鞘糖脂标准品在五分钟内分离，理论板数在640，000和740，000之间，检测限为约3 pM或240 ymol分析物注射到毛细管上。
Several glycosphingolipids were labeled with the fluorphore Bodipy-Fl and analyzed using capillary electrophoresis with laser-induced fluorescence detection. GM1-, LacCer-, and Cer-Bodipy-Fl were prepared through acylation using the N-hydroxysuccinimide ester of Bodipy-Fl. Several other glycosphingolipids including GT1a-, GD1a-, GM2-, GM3-, GD3-, and GlcCer-Bodipy-Fl were enzymatically synthesized. Micellar electrokinetic capillary chromatography with a TRIS/CHES/SDS/α-cyclodextrin buffer produced better separation than an established borate/deoxycholate/methyl-β-cyclodextrin buffer. The nine Bodipy-Fl-labeled glycosphingolipid standards were separated in under five minutes, theoretical plate counts were between 640,000 and 740,000, and the limit of detection was approximately 3 pM or 240 ymol analyte injected onto the capillary.
登录
查看更多内容
影响因子：
4.8
作者：
Gilbert, M;Brisson, JR;Wakarchuk, WW
通讯作者：
Wakarchuk, WW
DOI：
10.1016/0165-3806(83)90006-8
发表时间：
1983-01-01
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
FERRARI, G;FABRIS, M;GORIO, A
通讯作者：
GORIO, A
影响因子：
7.4
作者：
CHENG, YF;WU, SL;DOVICHI, NJ
通讯作者：
DOVICHI, NJ
DOI：
10.1016/j.jchromb.2008.06.001
发表时间：
2008-07-01
影响因子：
3
作者：
Goto-Inoue, Naoko;Hayasaka, Takahiro;Setou, Mitsutoshi
通讯作者：
Setou, Mitsutoshi
影响因子：
7.4
作者：
Essaka, David C.;White, John;Dovichi, Norman J.
通讯作者：
Dovichi, Norman J.