Chronic Mucosal Inflammation of the Gastric Cardia in Gastroesophageal Reflux Disease Is Not Regulated by FOXP3-Expressing T cells
Chronic Mucosal Inflammation of the Gastric Cardia in Gastroesophageal Reflux Disease Is Not Regulated by FOXP3-Expressing T cells
复制标题
胃食管反流病中贲门的慢性粘膜炎症不受表达 FOXP3 的 T 细胞的调节
DOI：
10.1007/s10620-009-0746-z
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发表时间：
1946
影响因子：
3.1
通讯作者：
Malfertheiner P.
中科院分区：
文献类型：
--
作者：
Kandulski A;Kuester D;Mönkemüller K;Peitz U;Roessner A;Malfertheiner P.
前言胃食管反流病（GERD）和幽门螺杆菌（Helicobacter pylori）均可引起贲门慢性炎症。调节性T细胞已被证明为幽门螺杆菌诱导的胃炎，而他们的作用还没有被研究在GERD.MethodsWe前瞻性地分析了FOXP 3的表达，各种调节性T细胞的标志物，以及TGF-β1和IL-10的粘膜转录水平。根据GERD（n= 22），对照组（n= 17）和幽门螺杆菌（n= 31）分层的70例患者的心脏活检中确定了RNA和蛋白质水平。结果GERD表现为慢性炎症，心脏粘膜中FOXP 3-mRNA减少（-84%），而幽门螺杆菌阳性患者FOXP 3基因表达增加25.1倍。这些结果得到了证实的调节细胞因子IL-10和TGF-β1，并通过免疫组化检测粘膜内FOXP 3表达的T cells.ConclusionChronic inflammatory at the cardia associated with either GERD or H. pylori differentiated about the presence of FOXP 3-expressing T cells.与幽门螺杆菌相反，表达FOXP 3的T细胞与GERD相关性心脏炎无关。
IntroductionChronic inflammation at the cardia occurs in gastroesophageal reflux disease (GERD), as well as in the presence ofHelicobacter pylori. Regulatory T cells have been demonstrated forH. pylori-induced gastritis, whereas their role has not been studied in GERD.MethodsWe prospectively analyzed the expression of FOXP3, a marker of various regulatory T cells, as well as the mucosal transcript levels of TGF-β1 and IL-10. RNA and protein levels have been determined in cardiac biopsies of 70 patients stratified according to GERD (n= 22), controls (n= 17), andH. pylori(n= 31).ResultsGERD presented with chronic inflammation and reduced FOXP3-mRNA in the cardiac mucosa (−84%), whereasH. pylori-positive patients revealed a 25.1-fold increase of FOXP3 gene expression. These results were verified by the regulatory cytokines IL-10 and TGF-β1, and by the immunohistochemical detection of intramucosal FOXP3-expressing T cells.ConclusionChronic inflammation at the cardia associated with either GERD orH. pyloridiffers concerning the presence of FOXP3-expressing T cells. In contrast toH. pylori, FOXP3-expressing T cells are not associated with GERD-associated carditis.
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影响因子：
9.8
作者：
Isomoto, H;Saenko, VA;Kohno, S
通讯作者：
Kohno, S
CD4+CD25+调节性T细胞的缺失与调节性T细胞的丧失相关，从而导致幽门螺杆菌感染小鼠的病理学增加
影响因子：
4.6
作者：
S. Raghavan;M. Fredriksson;A. Svennerholm;J. Holmgren;E. Suri‐Payer
通讯作者：
E. Suri‐Payer
DOI：
10.1084/jem.20060468
发表时间：
2006-07-10
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
通讯作者：
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胃食管反流病不会导致食管粘膜分泌性白细胞蛋白酶抑制剂表达的变化
影响因子：
2.1
作者：
Mönkemüller K;Kuester D;Weise S;Kropf S;Fry LC;Stahr A;Völkel S;Roessner A;Malfertheiner P.
通讯作者：
Malfertheiner P.
影响因子：
29.4
作者：
Harris, Paul R.;Wright, Shelton W.;Smith, Phillip D.
通讯作者：
Smith, Phillip D.