MicroRNA-183 family expression in hair cell development and requirement of microRNAs for hair cell maintenance and survival.
MicroRNA-183 family expression in hair cell development and requirement of microRNAs for hair cell maintenance and survival.
复制标题
DOI：
10.1002/dvdy.22591
复制
发表时间：
2011-04
影响因子：
2.5
通讯作者：
Soukup, Garrett A.
中科院分区：
文献类型：
--
作者：
Weston, Michael D.;Pierce, Marsha L.;Jensen-Smith, Heather C.;Fritzsch, Bernd;Rocha-Sanchez, Sonia;Beisel, Kirk W.;Soukup, Garrett A.
关键词：
MicroRNAs(MiRNAs)转录后抑制互补靶基因的表达，并有助于细胞分化。MiRNA-183家族成员(miR-183、miR-96和miR-182)在小鼠内耳和其他脊椎动物感觉器官的感觉细胞中协同表达。为了进一步检测毛细胞miRNA在小鼠内耳中的表达，我们分析了miR-183家族在野生型动物和各种神经感觉发育缺陷突变体中的表达。MIR-183家族成员的表达遵循神经感觉细胞的特点，在成熟的耳蜗毛细胞中表现出纵向(基底-顶端)的梯度，并在感觉神经元和大多数毛细胞中保持到成年期。在Atoh1-Cre转基因小鼠中，Dicer1条件敲除(CKO)导致的毛细胞miRNAs枯竭导致更多不同的基础-顶端基因表达谱，并最终导致毛细胞丧失。结果表明，毛细胞miRNAs抑制了耳蜗梯度基因的表达，是毛细胞维持和生存所必需的。
MicroRNAs (miRNAs) post-transcriptionally repress complementary target gene expression and can contribute to cell differentiation. The coordinate expression of miRNA-183 family members (miR-183, miR-96, and miR-182) has been demonstrated in sensory cells of the mouse inner ear and other vertebrate sensory organs. To further examine hair cell miRNA expression in the mouse inner ear, we have analyzed miR-183 family expression in wild type animals and various mutants with defects in neurosensory development. miR-183 family member expression follows neurosensory cell specification, exhibits longitudinal (basal-apical) gradients in maturating cochlear hair cells, and is maintained in sensory neurons and most hair cells into adulthood. Depletion of hair cell miRNAs resulting from Dicer1 conditional knockout (CKO) in Atoh1-Cre transgenic mice leads to more disparate basal-apical gene expression profiles and eventual hair cell loss. Results suggest that hair cell miRNAs subdue cochlear gradient gene expression and are required for hair cell maintenance and survival.
登录
查看更多内容
影响因子：
64.8
作者：
Baek, Daehyun;Villen, Judit;Shin, Chanseok;Camargo, Fernando D.;Gygi, Steven P.;Bartel, David P.
通讯作者：
Bartel, David P.
影响因子：
64.8
作者：
BenArie, N;Bellen, HJ;Zoghbi, HY
通讯作者：
Zoghbi, HY
DOI：
10.1073/pnas.0808175105
发表时间：
2008-11-25
影响因子：
11.1
作者：
Dabdoub, Alain;Puligilla, Chandrakala;Kelley, Matthew W.
通讯作者：
Kelley, Matthew W.
影响因子：
13.6
作者：
Hammond, SM
通讯作者：
Hammond, SM
DOI：
10.1002/ar.a.20306
发表时间：
2006-04-01
期刊：
ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY
影响因子：
--
作者：
Carey, J;Amin, N
通讯作者：
Amin, N