Intraspecific Transcriptome Variation and Sex-Biased Expression in Anopheles arabiensis.
Intraspecific Transcriptome Variation and Sex-Biased Expression in Anopheles arabiensis.
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DOI：
10.1093/gbe/evab199
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发表时间：
2021-09-01
影响因子：
3.3
通讯作者：
Ranz JM
中科院分区：
文献类型：
--
作者：
Jayaswal V;Ndo C;Ma HC;Clifton BD;Pombi M;Cabrera K;Couhet A;Mouline K;Diabaté A;Dabiré R;Ayala D;Ranz JM
按蚊种内转录变异的大小和功能模式，包括与疟疾传播相关的性别特异性特征背后的性别偏向基因的转录变异的大小和功能模式，仍然没有得到充分研究。因此，人们对表达水平的变化如何驱动这些物种的适应知之甚少。我们对来自布基纳法索的三个阿拉伯按蚊群体的雌性、雄性和幼虫转录组进行了测序。三分之一的基因在种群之间存在差异表达，通常以样本类型特异性的方式涉及杀虫剂抗性相关基因，并且雌性表现出最大数量的差异表达基因。在基因组水平上，与常染色体相比，X 染色体似乎缺乏差异表达的基因，含有倒位的染色体没有表现出此类基因富集的证据，而对群体分化的功能富集模式贡献最大的基因往往聚集在基因组中。此外，不同人群中性别表达比例的变异幅度在男性和女性偏向基因之间没有显着差异，除了一些群体中男性有限表达的基因比女性对应基因表现出更多的变异。事实上，无论是在男性还是女性中进行检测，偏向女性的基因都比偏向男性的基因表现出更大水平的群体间变异。除了揭示 An 转录变异的广泛适应性潜力之外。在阿拉伯树中，我们的研究结果表明，X 染色体上表达水平变化的进化率超过了常染色体上的变化率，同时指出偏向雌性的基因是阿拉伯树中变异最大的组成部分。阿拉伯树转录组。
The magnitude and functional patterns of intraspecific transcriptional variation in the anophelines, including those of sex-biased genes underlying sex-specific traits relevant for malaria transmission, remain understudied. As a result, how changes in expression levels drive adaptation in these species is poorly understood. We sequenced the female, male, and larval transcriptomes of three populations of Anopheles arabiensis from Burkina Faso. One-third of the genes were differentially expressed between populations, often involving insecticide resistance-related genes in a sample type-specific manner, and with the females showing the largest number of differentially expressed genes. At the genomic level, the X chromosome appears depleted of differentially expressed genes compared with the autosomes, chromosomes harboring inversions do not exhibit evidence for enrichment of such genes, and genes that are top contributors to functional enrichment patterns of population differentiation tend to be clustered in the genome. Further, the magnitude of variation for the sex expression ratio across populations did not substantially differ between male- and female-biased genes, except for some populations in which male-limited expressed genes showed more variation than their female counterparts. In fact, female-biased genes exhibited a larger level of interpopulation variation than male-biased genes, both when assayed in males and females. Beyond uncovering the extensive adaptive potential of transcriptional variation in An. Arabiensis, our findings suggest that the evolutionary rate of changes in expression levels on the X chromosome exceeds that on the autosomes, while pointing to female-biased genes as the most variable component of the An. Arabiensis transcriptome.
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DOI：
10.1073/pnas.2235649100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Attardo, GM;Higgs, S;Raikhel, AS
通讯作者：
Raikhel, AS
影响因子：
2.9
作者：
Awono-Ambéné, HP;Robert, V
通讯作者：
Robert, V
影响因子：
9.8
作者：
Baldini F;Gabrieli P;South A;Valim C;Mancini F;Catteruccia F
通讯作者：
Catteruccia F
DOI：
10.1111/evo.13176
发表时间：
2017-03
期刊：
Evolution; international journal of organic evolution
影响因子：
--
作者：
Ayala D;Acevedo P;Pombi M;Dia I;Boccolini D;Costantini C;Simard F;Fontenille D
通讯作者：
Fontenille D
影响因子：
7
作者：
Faucon F;Dusfour I;Gaude T;Navratil V;Boyer F;Chandre F;Sirisopa P;Thanispong K;Juntarajumnong W;Poupardin R;Chareonviriyaphap T;Girod R;Corbel V;Reynaud S;David JP
通讯作者：
David JP