Characterization by serial deletion competition ELISAs of HIV-1 V3 loop epitopes recognized by monoclonal antibodies.
Characterization by serial deletion competition ELISAs of HIV-1 V3 loop epitopes recognized by monoclonal antibodies.
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通过单克隆抗体识别的 HIV-1 V3 环表位的连续删除竞争 ELISA 进行表征。
DOI：
10.1016/0161-5890(96)00044-2
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发表时间：
1996
影响因子：
3.6
通讯作者：
Sokolowski,KA
中科院分区：
文献类型：
--
作者：
Seligman,SJ;Binley,JM;Gorny,MK;Burton,DR;Zolla-Pazner,S;Sokolowski,KA
通过对一系列四种小鼠mAb、一种人mAb和一种人Fab进行竞争ELISAs，对HIV-1包膜糖蛋白gp 120的V3环上抗体识别的位点进行表征。固相抗原由生物素-YNKRKRIHIGPGRAFYTTKN（来自gp 120 MN的V3环中心的序列）组成，应用于链霉亲和素包被的威尔斯孔。竞争抗原是两个系列的肽，其具有HIV-1 MN序列，每个序列在N或C末端连续缺失，但在另一末端保持恒定。对于每个系列，鉴定了在缺失末端处产生最小KD所需的氨基酸。表位定义为包括两个鉴定的氨基酸作为末端氨基酸的序列。对于报道的六种抗体，表位长度范围为7至14个氨基酸。使用环肽作为竞争性液相抗原表明了构象限制对假定的“线性”表位的影响。操作定义的表位比接触残基在其中的X-射线晶体结构已被确定的两个实例之一。基于具有连续缺失的竞争ELISA的当前研究中的表位长度的较长估计表明，非接触残基在表位定义和包括免疫原设计的功能应用中都是重要的。
Characterization of the sites recognized by antibody on the V3 loop of the envelope glycoprotein gp120 of HIV-1 was done by competition ELISAs on a series of four mouse mAbs, a human mAb and a human Fab. The solid-phase antigen consisted of biotin-YNKRKRIHIGPGRAFYTTKN, a sequence from the center of the V3 loop of gp120MN, applied to streptavidin-coated wells. Competing antigens were two series of peptides with the HIV-1MNsequence each serially deleted at either the N or C terminus but kept constant at the other terminus. For each series, the amino acid at the deleting end needed to give a minimum KDwas identified. The epitope was defined as the sequence including both of the identified amino acids as terminal amino acids. For the six antibodies reported, the epitope length ranged from seven to 14 amino acids. Use of a cyclic peptide as competing fluid-phase antigen suggested the influence of conformational constraints on presumed “linear” epitopes. The operationally-defined epitope was longer than the contact residues in one of two instances in which the X-ray crystallographic structure had been determined. The longer estimates of epitope length in the current study based on competition ELISAs with serial deletions suggest that non-contact residues are significant both in epitope definition and in functional applications including immunogen design.
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抗 gp120（人类免疫缺陷病毒中和抗体）识别的抗原决定簇的 NMR 图谱。
DOI：
10.1111/j.1432-1033.1995.0178l.x
发表时间：
1995
期刊：
European journal of biochemistry
影响因子：
--
作者：
A. Zvi;I. Kustanovich;D. Feigelson;R. Levy;M. Eisenstein;S. Matsushita;P. Richalet;M. H. Regenmortel;J. Anglister
通讯作者：
J. Anglister
影响因子：
2.9
作者：
CHANDRASEKHAR, K;PROFY, AT;DYSON, HJ
通讯作者：
DYSON, HJ
影响因子：
56.9
作者：
C. Y. Wang;D. Looney;M. Li;A. Walfield;J. Ye;B. Hosein;J. Tam;F. Wong-Staal
通讯作者：
C. Y. Wang;D. Looney;M. Li;A. Walfield;J. Ye;B. Hosein;J. Tam;F. Wong-Staal
影响因子：
4.4
作者：
M. Gorny;Jian-Yin Xu;S. Karwowska;A. Buchbinder;S. Zolla-Pazner
通讯作者：
M. Gorny;Jian-Yin Xu;S. Karwowska;A. Buchbinder;S. Zolla-Pazner
DOI：
10.1099/00221287-139-8-1729
发表时间：
1993-08-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
CHRISTODOULIDES, M;MCGUINNESS, BT;HECKELS, JE
通讯作者：
HECKELS, JE