Identification and functional analysis of beta-tubulin genes by site specific integrative transformation in Aspergillus nidulans
Identification and functional analysis of beta-tubulin genes by site specific integrative transformation in Aspergillus nidulans
复制标题
通过位点特异性整合转化构巢曲霉β-微管蛋白基因的鉴定和功能分析
作者：
John;Gambino;James A. Weatherbee;N. Ronald;Morris
我们从丝状真菌中克隆了两种不同的β -微管蛋白序列。每一种都被用于构建携带粗神经孢子虫pyr4基因的转化质粒。我们利用这些质粒将一株pyrg曲霉转化为尿苷原生菌。这两种质粒都能特异地整合到同源染色体序列中。然后，我们在遗传杂交中使用转化菌株来证明克隆的β -微管蛋白序列之一是benA β -微管蛋白基因，该基因编码β 1和β 2微管蛋白。另一个克隆的β -微管蛋白序列被证明是β - 3-微管蛋白的结构基因，并参与分生孢子的发育。这是第一次报道通过特定位点的基因破坏，整合重组在细粒曲霉。
We have cloned two different beta-tubulin sequences from the filamentous fungus Aspergillus nidulans. Each was used in the construction of transforming plasmids that carry the pyr4 gene of Neurospora crassa. We used these plasmids to transform a pyrG-strain of Aspergillus to uridine prototrophy. Both plasmids were shown to integrate site specifically into the homologous chromosomal sequences. We then used transformant strains in genetic crosses to demonstrate that one of the cloned beta-tubulin sequences was the benA beta-tubulin gene, which codes for the beta 1-and beta 2-tubulins. The other cloned beta-tubulin sequence was shown to be the structural gene for beta 3- tubulin by gene disruption and to participate in conidial development. This is the first report of a gene disruption by site specific, integrative recombination in Aspergillus nidulans.
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海胆肌动蛋白基因家族五个成员的特征。
DOI：
10.1016/0005-2787(81)90087-3
发表时间：
1981
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Overbeek,PA;Merlino,GT;Peters,NK;Cohn,VH;Moore,GP;Kleinsmith,LJ
通讯作者：
Kleinsmith,LJ
DOI：
10.1073/pnas.81.5.1470
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
YELTON, MM;HAMER, JE;TIMBERLAKE, WE
通讯作者：
TIMBERLAKE, WE
绒泡菌对苯并咪唑耐药性的遗传分析：β-微管蛋白基因的差异表达。
影响因子：
3.3
作者：
Burland,TG;Schedl,T;Gull,K;Dove,WF
通讯作者：
Dove,WF
绒泡菌微管蛋白基因家族的遗传学。
影响因子：
3.3
作者：
Schedl,T;Owens,J;Dove,WF;Burland,TG
通讯作者：
Burland,TG