The structure of a receptor with two associating transmembrane domains on the cell surface: integrin alphaIIbbeta3.
The structure of a receptor with two associating transmembrane domains on the cell surface: integrin alphaIIbbeta3.
复制标题
DOI：
10.1016/j.molcel.2009.02.022
复制
发表时间：
2009-04-24
期刊：
影响因子：
16
通讯作者：
Springer, Timothy A.
中科院分区：
文献类型：
--
作者：
Zhu, Jieqing;Luo, Bing-Hao;Barth, Patrick;Schonbrun, Jack;Baker, David;Springer, Timothy A.
具有单通跨膜（TM）结构域的完整受体的结构对于了解细胞外和细胞质结构域如何通过TM结构域调节缔合和信号传导。在这里开发并用糖蛋白验证。域，以及近光和均匀的二聚体的原理β中的JM LYS居住在界面中也具有重要作用。 βTM结构域显示了TM关联/解离如何调节整联蛋白信号传导。
Structures of intact receptors with single-pass transmembrane (TM) domains are essential to understand how extracellular and cytoplasmic domains regulate association and signaling through TM domains. A chemical and computational method to determine structures of the membrane regions of such receptors on the cell surface is developed here and validated with glycophorin. An integrin heterodimer structure reveals association over most of the lengths of the α and β TM domains, and that the principles governing association of hetero and homo TM dimers differ. A turn at the Gly of the juxtamembrane (JM) GFFKR motif caps the α TM helix, and brings the two Phe of GFFKR into the α/β interface. A JM Lys residue in β also has an important role in the interface. The structure is incompatible with previous NMR JM/cytoplasmic complex structures, and together with NMR structures of isolated α and β TM domains, shows how TM association/dissociation regulates integrin signaling. A joint ectodomain and membrane structure shows that substantial flexibility between the extracellular and TM domains is compatible with TM signaling.
登录
查看更多内容
影响因子：
2.9
作者：
Lau, Tong-Lay;Partridge, Anthony W.;Ulmer, Tobias S.
通讯作者：
Ulmer, Tobias S.
影响因子：
2.9
作者：
Duneau, Jean-Pierre;Vegh, Attila P.;Sturgis, James N.
通讯作者：
Sturgis, James N.
影响因子：
56.9
作者：
BRUNGER, AT;CLORE, GM;NILGES, M
通讯作者：
NILGES, M
影响因子：
56.9
作者：
MacKenzie, KR;Prestegard, JH;Engelman, DM
通讯作者：
Engelman, DM
影响因子：
14.9
作者：
Davis IW;Leaver-Fay A;Chen VB;Block JN;Kapral GJ;Wang X;Murray LW;Arendall WB 3rd;Snoeyink J;Richardson JS;Richardson DC
通讯作者：
Richardson DC