Sequence and expression of NUC1, the gene encoding the mitochondrial nuclease in Saccharomyces cerevisiae.
Sequence and expression of NUC1, the gene encoding the mitochondrial nuclease in Saccharomyces cerevisiae.
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NUC1（酿酒酵母中编码线粒体核酸酶的基因）的序列和表达。
DOI：
10.1093/nar/16.8.3297
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发表时间：
1988
影响因子：
14.9
通讯作者：
Zassenhaus,HP
中科院分区：
文献类型：
--
作者：
Vincent,RD;Hofmann,TJ;Zassenhaus,HP
介绍了酿酒酵母NUC1基因的DNA序列和表达研究。 NUC1 位点位于 X 染色体左臂的远端，编码线粒体中发现的主要核酸酶。推断的 NUC1 氨基酸序列预测该核酸酶是碱性的，富含脯氨酸，具有平均疏水性，初级翻译产物的分子量为 37,209 道尔顿。NUC1 的表达非常差，这与根据 DNA 序列确定的密码子使用偏差和我们之前确定的每个细胞的酶分子数一致。 NUC1mRNA 5'末端的作图表明，该mRNA具有长400个碱基的非翻译前导序列1n，其存在三个开放阅读框，每个开放阅读框由AUG起始。讨论了这些上游开放阅读框导致NUC1基因表达不良的可能性。
The DNA sequence and studies on the expression of theNUC1gene fromSaccharomyces cerevisiaeare presented. TheNUC1locus is located in the distal portion of the left arm of Chromosome X and encodes the major nuclease found in mitochondria. The inferred amino add sequence ofNUC1predicts that the nuclease is basic, rich in prolines, of average hydrophobicity, and has a molecular weight for the primary translation product of 37,209 daltons.NUC1is very poorly expressed, consistent with the codon usage bias determined from the DNA sequence and our previous determination of the number of enzyme molecules per cell. Mapping of the 5′ terminus of theNUC1mRNA reveals that the mRNA has a long 400 base untranslated leader 1n which are found three open reading frames, each initiated by an AUG. The possibility that these upstream open reading frames contribute to the poor expression of theNUC1gene is discussed.
构建缺乏线粒体核酸酶的酵母突变体。
影响因子：
14.9
作者：
Zassenhaus,HP;Hofmann,TJ;Uthayashanker,R;Vincent,RD;Zona,M
通讯作者：
Zona,M