Equipment Grant: Macromolecular Transport in Normal and Tumor Capillary Beds (Effects in Charge and Configuration)

本研究的总体目标是量化 分子 构型和电荷对正常大分子输运的影响 和肿瘤毛细血管床。 这些分子包括充分表征的 葡聚糖、免疫球蛋白和单克隆抗体，全部用 异硫氰酸荧光素（FITC）。 实验方法包括 生长正常（成熟肉芽）和肿瘤（VX 2癌）组织 在CMU常规制备的透明兔耳室中 实验室 一旦组织制备成熟，FITC标记 分子被注入动物的循环系统。 所得 分子的外渗被录像长达三小时 使用活体荧光显微镜技术， 实验室 由于光信号的强度非常低（即， 10-6英尺烛光），将购买一台高灵敏度的摄像机。 更高的灵敏度和摄像头分辨率可实现 要监测的血管的一小段。

The overall aim of this study is to quantify the effects of molecular configuration and charge on the transport of macromolecules in normal and tumor capillary-beds. These molecules include well characterized dextrans, immunoglobulins, and monoclonal antibodies, all tagged with fluorescein isothiocyanate (FITC). Experimental methods involve growing normal (mature granulation) and tumor (VX2 Carcinoma) tissues in a transparent rabbit ear chamber prepared routinely in the CMU laboratory. Once the tissue preparation is mature, FITC tagged molecules are injected into the animal's circulation. Resulting extravasation of molecules is video recorded for up to three hours using an intravital fluorescene microscopy technique developed in the laboratory. Since the intensity of the photo-signal is very low (i.e. 10-6 ft- candle), a highly sensitive video camera will be purchased. Increased sensitivity and camera resolution enable transport over a small segment of a blood vessel to be monitored..