Effect of Erythrocytes on Cellular Interactions Under Flow

血流下红细胞对细胞相互作用的影响

• 批准号: 9529673
• 负责人: Rakesh Jain
• 金额: $ 21.16万
• 依托单位: Massachusetts General Hospital
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9529673&HistoricalAwards=false
• 关键词: Effect; Erythrocytes; Cellular; Interactions; Under

9529673 Jain The binding of circulating cells in the blood to the vascular wall is a key step in many physiological processes, e.g., inflammation, wound healing, cancer metastasis, etc. Previous studies have neglected the impact of red blood cells (RBC's), a major blood component (up to 40% by volume) on the adhesion of these circulating cells to the endothelial vascular lining. Three key questions to be answered are: What effect do the RBC's have on the rates of cell "rolling" and binding of lymphocytes and cancer cells to endothelial cell monolayers? Are the observed effects biochemical and/or physical in nature? Can a theoretical framework be developed to model the RBC-enhanced adhesion? The main hypothesis is that the RBC's will enhance the adhesion of the cells to the endothelial lining. The hypothesis will be tested in a laminar flow chamber. The researchers are already familiar with the test chamber and the specified cells. ***

9529673 Jain血液中的循环细胞与血管壁的结合是许多生理过程中的关键步骤，例如，先前的研究忽略了红细胞（RBC）（主要血液成分（高达40体积%））对这些循环细胞与内皮血管衬里的粘附的影响。 需要回答的三个关键问题是： 红细胞对细胞增殖率有什么影响 淋巴细胞和癌细胞的“滚动”和结合， 内皮细胞单层 观察到的影响是生物化学的和/或物理的性质？ 能否建立一个理论框架来模拟RBC增强的粘附？ 主要假设是红细胞会增强细胞与内皮细胞的粘附。 将在层流室中检验该假设。 研究人员已经熟悉了测试室和指定的细胞。 ***