Protein Refolding in the Activation of Denatured Xylose Isomerase

变性木糖异构酶激活中的蛋白质重折叠

• 批准号: 8610844
• 负责人: George Tsao
• 金额: $ 7.41万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-15 至 1989-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8610844&HistoricalAwards=false
• 关键词: Protein; Refolding; Activation; Denatured; Xylose

A large percentage of protein products from genetically engineered cells exist in scrambled forms and are biologically nonfunctional. First, unfolding and then refolding these inactive products into active proteins is one of the most important problems of the new biotechnology. Hardly any bioprocess engineering research has ever been done in this area and, in fact, most engineers have not been aware of such difficulties until recently. This study will examine several related approaches to refold a model protein, xylose isomerase, after first unfolding. The objective is the development of bioprocessing techniques that will allow improved percentage yield of active products from scrambled proteins.

很大比例的蛋白质产品来自基因工程 细胞以杂乱的形式存在并且在生物学上是无功能的。 首先，将这些非活性产物展开，然后重新折叠成活性产物， 蛋白质是最重要的问题之一， 生物技术. 几乎没有任何生物过程工程研究 事实上，大多数工程师都没有意识到， 直到最近，这种困难。 本研究将探讨几个 相关的方法来重折叠模型蛋白质，木糖异构酶， 首先展开。 目标是生物加工的发展 将允许提高活性产物的百分比产率的技术 从杂乱的蛋白质中。