Analysis and Parameter Estimation of Immobilized Enzyme Reactions

固定化酶反应的分析和参数估计

• 批准号: 8611250
• 负责人: George Tsao
• 金额: $ 15万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-10-15 至 1989-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8611250&HistoricalAwards=false
• 关键词: Analysis; Parameter; Estimation; Immobilized; Enzyme

The newly-evolving area of biotechnology involves an attempt to use microorganisms to produce new products. Essential is the development of novel configurations of large bioreactors that utilize the microorganisms more efficiently than the reactors of the past which had to be run in batches and consisted of very dilute aqueous systems (and therefore had to be followed by very expensive separation sections). Immobilizing enzymes on a solid support structure permits continuous operation and is aimed at alleviating the first of the above limitations. The design of large scale bioreactors, utilizing such immobilized enzymes is in its infancy. This research is attempting to use methodology developed in heterogeneous chemical catalysis for such bioreactors. The system to be used for these experimental studies are immobilized enzyme systems which behave as pseudo-first-order reacting systems. The solid phase, in this care a bioparticle, (analogous to the catalyst in chemical systems) is where the reaction occurs. The specific systems that will be used include immobilized glucose isomerase, immobilized invertase, and immobilized lactase.

新发展的生物技术领域试图利用 微生物生产新产品。 关键是发展 大型生物反应器的新配置， 微生物比过去的反应器更有效， 分批运行，并由非常稀的含水系统组成（和 因此，必须随后进行非常昂贵的分离部分）。 将酶固定在固体支持结构上允许连续的 操作，旨在缓解上述第一个问题 局限性。 设计大规模的生物反应器，利用这种 固定化酶还处于起步阶段。 这项研究试图 使用多相化学催化中开发的方法， 生物反应器 用于这些实验研究的系统是 表现为假一级反应的固定化酶系统 系统. 固相，在此护理中为生物颗粒，（类似于 化学系统中的催化剂）是反应发生的地方。 的 将使用的具体系统包括固定化葡萄糖 异构酶、固定化转化酶和固定化乳糖酶。