Misaminoacylation in Protein Biosynthesis

蛋白质生物合成中的丙氨酰化

• 批准号: 8616411
• 负责人: Dieter Soll
• 金额: $ 27.35万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-15 至 1991-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8616411&HistoricalAwards=false
• 关键词: Misaminoacylation; Protein; Biosynthesis

A detailed investigation of the biosynthetic pathway leading from glutamate to delta-aminolevulinic acid, the earliest committed chlorophyll precursor in plants will be carried out. The topic is of current interest with the recent discovery of the involvement of a special chloroplast transfer RNA in this process. Using the techniques of molecular biology, protein biochemistry and enzymology, the genes for the enzymes and tRNAs involved in this process will be cloned and characterization in order to investigate in detail the mechanism of action and regulation of these molecules. The experimental systems will be the cyanobacterium Synechocystis 6803, Chlamydomonas reinhardtii, or barley. These studies should uncover novel principles regarding the role of RNA as a cofactor in metabolic conversions, and will be important to future programs in plant molecular biology.

对植物中最早的叶绿素前体——谷氨酸-氨基乙酰丙酸的生物合成途径进行了详细的研究。随着最近发现一种特殊的叶绿体转移RNA参与这一过程，该主题引起了当前的兴趣。利用分子生物学、蛋白质生物化学和酶学技术，克隆和鉴定参与这一过程的酶和trna的基因，以详细研究这些分子的作用机制和调控。实验系统将是蓝藻胞杆菌6803，莱茵衣藻或大麦。这些研究将揭示RNA在代谢转化中作为辅助因子作用的新原理，对未来植物分子生物学的研究具有重要意义。