Manipulation of Ice Nucleation Protein to Analyze how the Critical Temperatures of Ice Nuclei are Determined

操纵冰核蛋白来分析如何确定冰核的临界温度

• 批准号: 8701197
• 负责人: Gareth Warren
• 金额: $ 21.03万
• 依托单位: DNA Plant Technologies, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-01 至 1990-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8701197&HistoricalAwards=false
• 关键词: Manipulation; Ice; Nucleation; Protein; Analyze

Ice nuclei can be applied to promote the independent freezing of large numbers of water droplets, and to transduce molecular events into macroscopic signals. Ice nuclei of bacterial origin vary in their critical temperature for activity; the variability is problematic for practical applications, and its cause is not understood. In this research, the ice nucleation gene InaZ is manipulated and its products are analyzed to discover what governs the variability in critical nucleation temperature. Changes in protein concentration, caused by promoter substitutions, are correlated with nucleation frequency; this will indicate the molecularity of the assembly reaction for ice frequency: this will indicate the molecularity of the assembly reaction for ice nuclei over a range of critical temperatures. The subcellular locations of mutant InaZ proteins are determined and compared to their ice nucleation potentials, to reveal relationships between protein primary structure, localization, and function. Domains from heterologous proteins are substituted into the InaZ protein to further explore the requirements for nucleation at the higher end of the range of critical temperatures. The information obtained is utilized in the engineering of ice nucleation proteins with enhanced capacities for forming populations of ice nuclei with uniformly high critical temperatures. The goal of this research is to analyze how the critical temperatures of ice nucleic are determined. This is a thoughtfully concerned and focused research effort dealing with a significant phenomenon of great interest in biology. The implications for frozen food processing, snow making, arctic ice engineering, climate modification and frost control are infinite.

冰核可以应用于促进独立 大量水滴的冻结， 分子事件转化为宏观信号。 冰核 细菌起源的临界温度不同， 活动;可变性对于实际应用是有问题的。 应用，其原因尚不清楚。 在这 研究，冰核基因InaZ被操纵，其 分析产品，以发现是什么控制了变化 在临界成核温度。 蛋白质变化 浓度，由启动子取代引起的，是相关的 与成核频率;这将表明 冰频率的组装反应：这将表明 冰核组装反应的分子性 临界温度范围。 亚细胞定位 测定突变InaZ蛋白并与它们的冰 成核势，揭示蛋白质之间的关系 一级结构定位和功能 结构域 将异源蛋白质取代到InaZ蛋白质中， 进一步探索在较高的成核要求 临界温度范围的终点。 的信息 在冰成核工程中得到了应用 具有增强的形成细胞群的能力的蛋白质 具有均匀高临界温度的冰核。 这项研究的目的是分析如何关键 确定冰核的温度。 这是一 经过深思熟虑的关注和集中的研究工作， 生物学中一个很重要的现象 的 对冷冻食品加工、造雪、北极的影响 冰工程，气候改造和霜冻控制， 无限的。