Mammalian Aminoacyl-tRNA Synthetase Complex

哺乳动物的高分子量氨基酰基- trna合成酶与多酶复合物相关，与低等生物的游离可溶性酶相反。该奖项的目标是阐明这些复杂实体的结构和功能，并建立控制蛋白质-蛋白质和蛋白质- rna相互作用的原则。计划进行三条调查。首先分析了合成酶复合物与蛋白质生物合成机制的结构和功能相互作用。第二步是通过分子克隆和相应cdna的测序来确定复合体中单个合成酶和相关蛋白的结构。最后是开发一种蛋白质足迹方法来确定参与蛋白质-蛋白质和蛋白质- rna相互作用的结构元件。超分子蛋白- rna复合物在活细胞的功能中起着极其重要的作用。这些研究的结果将使我们更好地理解这些复合物，特别是合成酶复合物是如何在哺乳动物和其他生命系统中组装和发挥作用的。

Mammalian high molecular weight aminoacyl-tRNA synthetases are associated in a multi-enzyme complex in contrast to the free soluble enzymes in lowere organisma. The objectives of this award are to elucidate the structure and function of these complex entities and to establish the principles which govern the protein-protein and protein-RNA interactions. Three lines of inquiry are planned. The first is analyze the structural and functional interactions of the synthetase complex with the protein biosythetic machinery. The second is to determine the structures of the individual synthetases and associated proteins in the complex through molecular cloning and sequencing of the respective cDNAs. The last is to develop a protein foot-printing method to determine the structural elements involved in the protein-protein and protein-RNA interactions. Supramolecular protein-RNA complexes are extremely important in the function of living cells. The results of these studies will lead to a better understanding of how such complexes in general, and the synthetase complex in particular, are assembled and endabled to function in mammalian and other living systems.