Symbiotic Nitrogen Fixation -- A Developmental Pathway

共生固氮——一条发展途径

• 批准号: 8703297
• 负责人: Ann Hirsch
• 金额: $ 9万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1990-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8703297&HistoricalAwards=false
• 关键词: Symbiotic; Nitrogen; Fixation; Developmental; Pathway

The aims of the proposal are to investigate the sequence of events involved in the early stages of nodule development using specific Rhizobium mutants that induce the formation of nodules on alfalfa roots which are arrested at distinct stages of their development. The PI hypothesizes that infection thread formation is the second signal for nodulin gene expression, and that the nod and exo genes interact in the early stages of nodule formation. Dr. Hirsch is proposing a multi-faceted approach utilizing techniques of plant tissue culture, genetics, molecular biology and electron microscopy. Both bacterial genes and plant genes are involved in the formation of a nitrogen fixing root nodule. Understanding the roles of these different genes in nodulation is essential if we are to transfer the nitrogen fixing capability into nonleguminous plants.

该提案的目的是利用特定的根瘤菌突变体来调查根瘤发育早期阶段所涉及的事件顺序，这些突变体可以诱导紫花苜蓿根瘤的形成，而根瘤在其发育的不同阶段被阻止。PI假设侵染线的形成是根瘤蛋白基因表达的第二信号，并且nod和exo基因在根瘤形成的早期阶段相互作用。赫希博士提出了一种利用植物组织培养、遗传学、分子生物学和电子显微镜技术的多方面方法。细菌基因和植物基因都参与了固氮根瘤的形成。如果我们要将固氮能力转移到非豆科植物中，了解这些不同基因在结瘤中的作用是至关重要的。